EcoRV completely encircles bound DNA with two loops, forming the entry and exit gate for the DNA substrate. These loops were crosslinked generating CL-EcoRV which binds and releases linear DNA only slowly, because threading linear DNA into and out of the DNA-binding 'tunnel' of CL-EcoRV is not very effective. If the crosslinking reaction is carried out with a circular bound DNA, CL-EcoRV is hyp...

The aim of this experimental study is two-dimensional investigation of cargo behavior after separation which is carried out in the subsonic wind tunnel. Given that the real separation occurs in three-dimension, the results of this study may be widely used in numerical aerodynamic studies for verification of new computational methods and they can be used as the reference results in this area. So...

Minimal Change disease (MCD) is the most common cause of Nephrotic Syndrome (NS) in children accounting for 70 to 90% of cases under the age of 10 years and 50% in older children.In adults MCD is found in 10 to 15% of cases with primary nephrotic syndrome. Most patients with MCD remit with steroids.1 Remission is defined as absence of proteinuria (urine albumin nil or trace on 3 conservative da...

This paper explored the effect of building shape on the wind-induced response of a structure through a comprehensive investigation of wind tunnel studies performed at Rowan Williams Davies and Irwin, Inc. (RWDI). The study focused on buildings with foot prints of square, circular, triangular, rectangular and elliptical shapes. Seed buildings were selected from an inventory of structures previou...

To solve the problem of a potential void near shallow rectangular masonry-concrete power tunnel, ground-penetrating radar (GPR) detection technology is used and multiple GPR lines are placed to detect concealed void. The images circular square voids obtained, which can help increase accuracy identification. models that cover 9 created investigate how various forms positions will affect internal...

The paper is focused on the assessment of seismic fragility curves for circular tunnels under moderate to severe earthquakes with aim improving reliability risk underground infrastructural networks. Non-linear two-dimensional dynamic analyses were performed different tunnel and soil configurations by using finite-difference method implemented in Flac2D software. applied input motions selected c...

In this study, the aerodynamic sound generated from the inclined circular cylinder was investigated experimentally and numerically. The generated sound and a coherent length of velocity fluctuation along the cylinder axis were measured by a low-noise wind tunnel with endplates. The sound and the flow in wake were also simulated by Large Eddy Simulation and Lighthill-Curle’s equation. We got the...