Introduction: Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells resulting in the increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. The research evaluated the cytotoxic effects of hydro-alcoholic extracts of Lepidium Sativum (Cress plant) shoots before and after flowering on K562 cell line as a...

Chronic Myeloid Leukemia (CML) is effectively treated with Tyrosine Kinase Inhibitors (TKI) such as Imatinib Mesylate (IM) targeted against BCR-ABL, however, several mathematical models and ex vivo-examinations suggested that IM does not eradicate CML stem cells. We previously reported the investigation of residual CML diseases during TKI treatment using FACS-sorting and quantitative RT-PCR of ...

This study shows for the first time that 1,25-dihydroxyvitamin D3[1,25-(OH)2D3] and two fluorinated analogues of 1,25-(OH)2D3 [24,24-F2-1,25-(OH)2D3 and 26, 26, 26, 27, 27, 27-F6-1,25-(OH)2D3] induce macrophage differentiation of human normal and leukemic myeloid stem cells. The addition of either 1,25-(OH)2D3 or one of the two fluorinated analogues of 1,25-(OH)2D3 at concentrations as low as 1...

Nowadays, more than 90% of patients affected by chronic myeloid leukemia (CML) survive with a good quality of life, thanks to the clinical efficacy of tyrosine kinase inhibitors (TKIs). Nevertheless, point mutations of the ABL1 pocket occurring during treatment may reduce binding of TKIs, being responsible of about 20% of cases of resistance among CML patients. In addition, the presence of leuk...

follicular dendritic cells or dendritic reticulum cells are important components of the immune system essential for antigen presentation. malignancies arising from these cells are uncommon and the first case was reported in 1986. the most common sites of follicular dendritic cell sarcomas are lymph nodes, especially cervical, axillary and mediastinal regions, but extranodal sites including head...

Objective: Lymphoid enhancer-binding factor-1 (LEF1) is one of the key regulators lymphocyte proliferation and its aberrant expression a prognostic factor for lymphoid or myeloid malignancies. In this study, we focused on LEF1 isoforms in several hematological malignancies found tissue-specific differential full-length (FL)-LEF1 gene tumor suppressor (∆LEF1) variant. Material Method: Fifty-thre...

Leukemia progression and relapse is fueled by leukemia stem cells (LSC) that are resistant to current treatments. In the progression of chronic myeloid leukemia (CML), blast crisis progenitors are capable of adopting more primitive but deregulated stem cell features with acquired resistance to targeted therapies. This in turn promotes LSC behavior characterized by aberrant self-renewal, differe...

Inherited or sporadic heterozygous mutations in the transcription factor GATA2 lead to a clinical syndrome characterized by non-tuberculous mycobacterial and other opportunistic infections, a severe deficiency in monocytes, B cells and natural killer cells, and progression from a hypocellular myelodysplastic syndrome to myeloid leukemias. To identify acquired somatic mutations associated with m...