Static light-scattering (LS) detection can determine the molecular weight (MW) of polymers eluted with size-exclusion chromatography (SEC) without using any standards when the differential refraction index (RI) of solutes are obtained. On the other hand, the noisy chromatographic signal peak acquired using a static LS detector often causes difficulty in peak-width recognition. This disadvantage...

Leaves from Panax ginseng Meyer (Korean origin and Chinese origin of Korean ginseng) and P. quinquefolius (American ginseng) were harvested in Haenam province, Korea, and were analyzed to investigate patterns in major metabolites using HPLC-based metabolic profiling. Partial least squares discriminant analysis (PLS-DA) was used to analyze the HPLC chromatogram data. There was a clear separation...

In order to establish the marker constituents of the natural antioxidant food-additive Eucalyptus leaf extract, the UV-absorbing constituents of two eucalyptus leaf extracts registered as food additives (eucalyptus A and B) were investigated. Several major peaks on the reversed-phase HPLC chromatogram of eucalyptus A were characterized as gallic acid, ellagic acid, 3-O-beta-D-glucuronides of qu...

The unidentified fluorescence develop in body fluids during uremia and cirrhosis. We have used "hydrophobic" HPLC to profile these fluorescence in both 10 uremic hemodialyzates and 5 cirrhosis urines, and studied further to characterize the fluorescence and to gain more information of uremic fluorescent middle molecules. Chromatograms indicate evidently that the fluorescent substances are prese...

Corydalis yanhusuo is used widely for the treatment of gastralgia, costalgia and dysmenorrhea in Chinese medicine. The alkaloid is the main active ingredient of C. yanhusuo. Response surface methodology was applied to optimize the separation and purification process for alkaloids by AB-8 resin-packed chromatogram column. The optimal conditions were found to be as follows: height-diameter ratio ...

To characterize organic nitrogen present in cattle manure compost, a hot-water extract of the compost along with an acid-insoluble (humic acid-like) fraction either treated with alkali or laccase or untreated were separated by size-exclusion high-performance liquid chromatography (SE-HPLC). Nitrogen was then detected by chemiluminescent nitrogen detection (CLND), and humic acid-like substances ...

In absorption chromatography, fused peaks may arise due to incomplete resolution of two or more eluting species. In figure 1, the chromatogram of an inorganic multicomponent polluting substance, acquired by means of a Dionex Ionic chromatographic system, is shown. The problem consists in quantitative evaluation of fluorine content, whose retention time is 3 mn, on the basis of the measured chro...

High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated Hb...