Food intake and body weight are determined by a complex interaction of regulatory pathways. To elucidate the contribution of the endogenous peptide cholecystokinin, mice lacking functional cholecystokinin-A receptors were generated by targeted gene disruption. To explore the role of the cholecystokinin-A receptor in mediating satiety, food intake of cholecystokinin-A receptor-/- mice was compar...

This study was undertaken to determine the role of cholecystokinin in pancreatic enzyme secretion stimulated by bombesin and a meal by (a) comparing the pancreatic enzyme output during bombesin infusion and after a meal to output during caerulein infusion and (b) comparing the inhibitory effect of the cholecystokinin-receptor antagonist lorglumide (CR-1409) on enzyme output in response to bombe...

We studied the effect of cholecystokinin (CCK-8) and yohimbine-alpha-2-adrenoreceptor antagonist administered locally into lateral reticular formation (LRF) on the bioelectrical response to the pain stimulation. The experiments were carried out on 8 conscious rabbits with bilaterally implanted electrodes into: motor-sensory cortex, ventro-postero-lateral thalamic nuclei, hippocampus and LRF. No...

A series of phenoxyacetanilide derivatives was synthesized and their antagonist activities for human gastrin/cholecystokinin (CCK)-B and CCK-A receptors were evaluated. Among the compounds synthesized, 2-[3-[3-[N-[2-(N-methyl-N-phenylcarbamoylmethoxy)phenyl]-N-(N-meth yl-N- phenylcarbamoylmethyl)carbamoylmethyl]-ureido]phenyl]acetic acid (20i, DA-3934) exhibited high affinity for gastrin/CCK-B ...

Streptomyces sp. NR0489 produces tetronothiodin, a novel brain-type cholecystokinin receptor antagonist. This species was differentiated from its related species S. gelaticus, S. griseolus and S. hydrogenans on the basis of their cultural characteristics, such as the utilization of carbohydrates and the presence or absence of various enzymatic activities. We applied the DNA-DNA hybridization me...

Cholecystokinin (CCK) is an amino acid peptide. It has been isolated from porcine duodenum. CCK is known to regulate like anxiety, schizophrenia and depression by acting on CCKB receptor. CCK-A receptors are localized in the pancreas, gallbladder, pylorus, intestine and the vagus nerve. CCK is administered peripherally and it inhibits food consumption in humans and other many other species. CCK...

N-(7-chloro-1-methyl-2-oxo-5-phenyl-2,3-dihydro-1H-benzo[e][1,4]diazepin-3-yl)thiophene-2 carboxamide-[14C-carboxy] was prepared as part of a 6-step sequence from thiophene-2-carbonitrile -[cyano-14C] as a keysynthetic intermediate which has been synthesized from 2-iodothiophene and zinc [14C]-cyanide in thepresence of tetrakis (triphenylphosphine) palladium.

In order to produce antibodies for the radioimmunoassay of cholecystokinin the following animals were immunized: 10 rabbits and 3 guinea pigs with cholecystokinin 1—33 coupled to bovine serum albumin, 9 rabbits and 2 guinea pigs with uncoupled cholecystokinin 1-33, 5 rabbits with cholecystokinin 10-20 coupled to bovine serum albumin and 4 rabbits with sulphated cholecystokinin 26-33 coupled to ...

Administration of cholecystokinin was recently found to attenuate opiate analgesia. In the present study, the role of endogenous cholecystokinin in opiate analgesia was examined. Endogenously released cholecystokinin was sequestered by antibodies to cholecystokinin developed in response to an active immunization procedure. Morphine analgesia was potentiated and prolonged in rats immunized again...