Poly(3-hydroxybutyrate) (PHB)/chitosan electrospun scaffold was recently prepared for cartilage tissue engineering purpose. The drawback of this scaffold was its low mechanical properties. This study was carried out to see if addition of multi-wall carbon nanotubes (MWNTs) to PHB/chitosan polymeric blend can show better mechanical and structural properties. To do this, three different amounts o...

Chitosan and gelatin were used as polymer scaffolds for cartilage tissue engineering. The scaffold was a biodegradable drug delivery system diclofenac sodium to treat defects on osteoarthritis (OA). materials composed of sodium, chitosan, gelatin, cross-linking agent-glutaraldehyde (GTA) form scaffold. purpose this study investigate the effect GTA concentration variations (0.00%; 0.25%; 0.50%; ...

The aim of this study was to develop a porous chitosan scaffold with long-acting drug release as an artificial dressing to promote skin wound healing. The dressing was fabricated by pre-freezing at different temperatures (-20 and -80 °C) for different periods of time, followed by freeze-drying to form porous chitosan scaffolds with different pore sizes. The chitosan scaffolds were then used to ...

For blood vessel tissue engineering, an ideal vascular graft should possess excellent biocompatibility and mechanical properties. For this study, a elastic material of poly (L-lactic acid-co-ε-caprolactone) (P(LLA-CL)), collagen and chitosan blended scaffold at different ratios were fabricated by electrospinning. Upon fabrication, the scaffolds were evaluated to determine the tensile strength, ...

Engineered bone substitutes are being extensively explored in response to growing demand. However, the angiogenesis that occurs during bone formation is often overlooked in scaffold design. In this novel study, we incorporated two small interfering RNAs (siRNAs), ie, small interfering RNA targets casein kinase 2 interaction protein 1 (siCkip-1) and small interfering RNA targets soluble VEGF rec...

Bone Marrow mesenchymal stem cells can be induced to differentiate into osteoblasts to regenerate damaged bone tissue using tissue engineering techniques. In this study, we examine the use of chitosan scaffolds with double pore structure prepared by an innovative method that combines freeze gelation (that produces micropores) and particle leaching out technique (that produces interconnected sph...

Generally, the lysozyme degradation on chitosan (CTS) is slower than that on chitin (CT). The CTS can be fabricated in scaffold form but it is difficult to fabricate CT scaffold under mild conditions. The method for the preparation of scaffold from N-acetylated CTS (N-CTS) was investigated in this research. By using this method, the scaffolds could be fabricated chitosan to chitin with the degr...

OBJECTIVE To develop a chitosan-based scaffold and carry out a complete comprehensive study encompassing optimization of exact chitosan strength, product characterization, toxicity evaluation, in vitro validation in cell culture experiments, and finally in vivo efficacy in animal excision wound model. MATERIALS AND METHODS Developed chitosan scaffolds (CSs) were optimized for tissue engineeri...

We report the preparation of a hybrid chitosan/silica three-dimensional (3D) scaffold loaded simultaneously with two model hydrophilic substances, ibuprofen sodium salt and erioglaucine disodium salt. The first substance is entrapped in situ during chitosan submillimetric beads by ionotropic gelation triphosphate, while latter post-loaded formation. Controlled release experiments carried out un...