Dopamine and to a much greater extent, 6-hydroxydopamine reduced the growth of the mouse neuroblastoma cell population in vitro but did not induce morphological differentiation. The growth inhibition at a lower drug concentration was primarily due to inhibition of cell multiplication, which was reversible. L-Dopa, norepinephrine, epinephrine, 3,4-dihydroxyphenylacetic acid, or homovanillic acid...

Background A large number of recent publications demonstrate the effect of cell culture media on post-translational modifications of recombinant proteins [1]. In this study, aiming to extend the toolbox of media design beyond the commonly known media components, a large variety of cell culture compatible chemical components such as sugars in industrial relevant Chinese hamster ovary cell lines ...

Background:  With one-third of the world’s population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should s...

Background: Human chorionic gonadotropin (hCG) is a member of glycoprotein hormones family consist of two different non-covalently heterodimeric chains: alpha and beta subunits with 92 and 145 amino acids respectively. This hormone plays an important role in human reproduction and physiology especially for maintenance of the corpus luteum during the first months of pregnancy Materials and Metho...

Chinese hamster ovary (CHO) cells have been used widely in the pharmaceutical industry for production of biological therapeutics including monoclonal antibodies (mAb). The integrity of the gene of interest and the accuracy of the relay of genetic information impact product quality and patient safety. Here we employed next-generation sequencing, particularly RNA-seq, and developed a method to sy...

Background Chinese hamster ovary (CHO) cells have become dominant host cells in the biopharmaceutical industry due to their capacity for proper protein folding, assembly and posttranslational modifications. However, low specific productivity (qp) places limitations on yields obtained from mammalian host cells. MicroRNAs (miRNAs), a novel class of short, non-coding RNAs which negatively regulate...

Background Sodium butyrate (NaBu) is not only known to inhibit proliferation but also to increase the specific productivity in cultivation of Chinese hamster ovary (CHO) cells [1] – the most commonly used mammalian cell line for pharmaceutical protein production [2]. So far, little is known about the underlying mechanisms and genes that are affected by butyrate treatment. Besides the proteomic ...

Background: The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development however, their role on metabolic pathway is not well understood. Methods: In the present study, the effect of different...

For the first time, a patient with asymptomatic QT interval prolongation was found to carry missense mutation c.277G>A (p.Val93Ile) in KCNJ2 gene, described previously only as cause of familial form atrial fibrillation. The corresponding amino acid substitution introduced into plasmid encoding Kir2.1 channel and mutant gene expressed Chinese hamster ovary cells (CHO-K1) evaluate effect on IK1 c...