We have established a PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with primers specific to adhesin subunit gene. The minimum amount of H. pylori DNA that could be detected was 0.4 pg. Seventy-two antral biopsy specimens were taken from patients with gastritis and/or duodenal ulcer. H. pylori was found in 58.3%, 44.4% and 54.2% of patients according to the resu...

Inorganic Cr(III) and Cr(VI) have contrasting biological, geochemical and toxicology effects. Cr(III) is considered as an essential species for the proper functioning of living organisms but Cr(VI) is toxic for the biological systems. An off-line speciation method using Chelex-100 has been practiced for speciation to Cr(III) and Cr(VI) from surface waters of rivers. The underlying principal of ...

BACKGROUND Few studies comparing multiple methods for DNA extraction from dried blood spots (DBS) on filter paper for PCR targeting the Plasmodium genome have been done. METHODS Frequently-used methods for DNA extraction from DBS using Chelex-100, InstaGene Matrix, QIAamp DNA Mini Kit and TE buffer were compared on a dilution series of a standardized Plasmodium falciparum positive sample. The...

BACKGROUND Dried blood spots (DBS) are a convenient and inexpensive method for biobanking. Although many countries have established population-based DBS biobanks from neonatal screening programs, the quality and usefulness of DNA from DBS have not been extensively assessed. METHODS We compared 4 common DNA extraction methods (Qiagen, EZNA, Chelex 100, and alkaline lysis) in a pilot study usin...

The "quantitative" ChIP, a tool commonly used to study protein-DNA interactions in cells and tissue, is a difficult assay often plagued with technical error. We present, herein, the process required to merge multiple protocols into a quick, reliable and easy method and an approach to accurately quantify ChIP DNA prior to performing PCR. We demonstrate that high intensity sonication for at least...

DNA extraction from paraffin-embedded tissue (PET) is a critical step for many molecular techniques. Several protocols have been carried out for this objective according to the literature. In the present study, the performances of three DNA extraction methods from PET were compared to establish the optimal protocol for our laboratory. Ten lymph nodes from ten patients dying of AIDS were investi...

Asymptomatic Plasmodium infection is a new challenge for public health in the American region. The polymerase chain reaction (PCR) is the best method for diagnosing subpatent parasitemias. In endemic areas, blood collection is hampered by geographical distances and deficient transport and storage conditions of the samples. Because DNA extraction from blood collected on filter paper is an effici...

PCR-based analysis of skeletonized human remains is a common aspect in both forensic identification as well Ancient DNA research. In this, areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality recovered presence inhibitory substances samples from excavated remains. To enable amplification based remains, development optimized extractio...