A simple spectrophotometric method for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin with variamine blue is presented. The determination is based on the hydrolysis of β-lactam ring of cephalosporins with sodium hydroxide which subsequently reacts with iodate to liberate iodine in acidic medium. The liberated iodine oxidizes variamine blue to violet colored species of m...

Variability in valacyclovir bioavailability and the potential for cephalexin-valacyclovir interaction were evaluated. The intraindividual acyclovir area under the concentration-time curve (AUC) varied minimally, whereas interindividual differences were substantial. Coadministration of the human peptide transporter 1 (hPEPT1) substrates valacyclovir and cephalexin minimally reduced the acyclovir...

The antibiotic cephalexin [systematic name: D-7-(2-amino-2-phenylacetamido)-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid] forms a range of isomorphic solvates, with the maximum hydration state of two water molecules formed only at high relative humidities. The water content of the structure reported here (C(16)H(17)N(3)O(4)S.1.9H(2)O) falls just short of this configurati...

The application of reverse phase high-performance liquid chromatography to the separation and analysis of cephradine and cephalexin is demonstrated. The procedure has been applied to chemicals, pharmaceutical formulations and reaction solutions. The preparation of samples is simple and rapid. Chromatographic conditions are described for both pellicular and small particle columns. The feasibilit...

CEPHALEXIN, a new semi-synthetic antibiotic derived from cephalosporin C, has the same basic nucleus and the same range of activity as its predecessors, cephalothin and cephaloridine but has the advantage that it can be given orally. As this drug is relatively new, few side-effects have been described. The following case report is of a boy, aged 14 years, who developed acute intravascular haemo...

Microbial growth inhibition assays are used to detect antimicrobial residues in meat. Postscreening methods may facilitate the selection of an appropriate chemical confirmatory method. Two such methods, the Charm II radioreceptor assay and High Voltage Electrophoresis (HVE), were evaluated for the detection of incurred β-lactam residues in pigmeat. Pig liver, kidney and muscle containing penici...

OBJECTIVES To determine the effectiveness of a single perioperative prophylactic 2 g dose of cephalexin in preventing surgical site infection (SSI) following excision of skin lesions from the lower limb. DESIGN Prospective double-blinded placebo-controlled trial testing for difference in infection rates. SETTING Primary care in regional North Queensland, Australia. PARTICIPANTS 52 patient...

Penicillin-binding protein 3 (PBP3; also called FtsI) is a transpeptidase that catalyzes cross-linking of the peptidoglycan cell wall in the division septum of Escherichia coli. To determine whether the catalytic activity of PBP3 is activated during division, we assayed acylation of PBP3 with three beta-lactams (cephalexin, aztreonam, and piperacillin) in growing cells. Acylation of PBP3 with c...