نتایج جستجو برای: cell patch clamp

تعداد نتایج: 1724747  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علم و فرهنگ - پژوهشکده رویان 1388

مقدمه : اگرچه سلول های بنیادی جنینی خودنوزا بوده و توان تمایز به انوع سلول های موجود در بدن را دارند ولی تمایز جهت دار آنها به یک نوع سلول خاص، با یک شیوه خاص، بعنوان چالشی است که هنوز باقی مانده است. از طرفی، چنین تمایزی در موجود زنده در یک محیط سه بعدی روی می دهد، لذا استفاده از داربست هایی که بتواند چنین وضعیتی را برای تمایز سلول ها فراهم آورد ضروری به نظر می رسد. روش ها: ما پس از کشت سلول ...

Journal: :Cold Spring Harbor protocols 2009
Jens Eilers Arthur Konnerth

This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction (e.g., Lucifer yellow); ion-sensitive indicator ...

Journal: :Current drug discovery technologies 2004
A Brueggemann M George M Klau M Beckler J Steindl J C Behrends N Fertig

Unlike the genomics revolution, which was largely enabled by a single technological advance (high throughput sequencing), rapid advancement in proteomics will require a broader effort to increase the throughput of a number of key tools for functional analysis of different types of proteins. In the case of ion channels -a class of (membrane) proteins of great physiological importance and potenti...

2017
Birgit Goversen Nadine Becker Sonja Stoelzle-Feix Alison Obergrussberger Marc A. Vos Toon A. B. van Veen Niels Fertig Teun P. de Boer

An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays. The benefits of this cell source are clear; drugs can be tested in vitro on human cardiomyocytes, with patient-specific genotypes if needed, and differentiation efficiencies are generally ...

2017
Arnaud Billet Lionel Froux John W. Hanrahan Frederic Becq

The chloride (Cl-) channel cystic fibrosis transmembrane conductance regulator (CFTR) is defective in cystic fibrosis (CF), and mutation of its encoding gene leads to various defects such as retention of the misfolded protein in the endoplasmic reticulum, reduced stability at the plasma membrane, abnormal channel gating with low open probability, and thermal instability, which leads to inactiva...

2012
Manabu Kubokawa

Ion channels are well-known functional proteins, and their activity can be directly estimated by monitoring ion currents through channel proteins using the patch-clamp technique (Hamill et al., 1981), which is a fine tool for the investigation of not only ion channels, but also the membrane current and membrane potential of the whole cell. Many investigators have applied the patch-clamp techniq...

Journal: :Moscow University Biological Sciences Bulletin 2021

For the first time, a patient with asymptomatic QT interval prolongation was found to carry missense mutation c.277G>A (p.Val93Ile) in KCNJ2 gene, described previously only as cause of familial form atrial fibrillation. The corresponding amino acid substitution introduced into plasmid encoding Kir2.1 channel and mutant gene expressed Chinese hamster ovary cells (CHO-K1) evaluate effect on IK1 c...

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