A combined cell culture enzyme-linked immunosorbent assay (CCC-ELISA) was developed for measuring the neutralizing antipoliovirus antibodies in human sera. The binding of different concentrations of each of the three poliovirus types to BGM cells in the presence and absence of a constant dilution from each test and reference serum was measured in the CCC-ELISA. The titers of the viruses neutral...

Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi...

We aimed to quantify the biological effects of an innovative Pickering emulsion coencapsulating immunosuppressive drug (e.g.cyclosporinA) in biodegradable and biocompatible PLGA nanoparticles, with anti-inflammatory (e.g.calcitriol) oil phase, on T-cells keratinocytes vitro a murine model psoriasis establish proof concept efficacy vivo. were isolated from healthy volunteer’ blood or skin explan...

Porcine eperythrozoonosis is a disease with worldwide distribution caused by the unculturable hemotrophic bacterium Mycoplasma suis. Current serological testing utilizes crude M. suis antigens purified from the blood of experimentally infected pigs. These antigens show high variability and are restricted to specialized laboratories. We evaluated a novel serological assay based on two recombinan...

INTRODUCTION Several immunological methods are used to determine the serum antinuclear antibodies (ANA). Indirect immunofluorescence assay (IFA) with tissue slices or HEp-2 cells is the standard technique considered the gold standard for their screening. Serum-free McCoy-Plovdiv cell line may also be used as substrate for IFA. Another method for detection of total and specific ANA is the enzyme...

Purified coronavirus, detergent extracts of purified coronavirus, and virus-infected Madin-Darby bovine kidney cells were evaluated as antigen substrates in enzyme-linked immunosorbent assay (ELISA) and passive hemagglutination systems. Only detergent-extracted and -unextracted, purified viruses were reactive as antigen substrates in ELISA, whereas all three antigen preparations could be used f...

MJ Martin, Met Office, Exeter, UK. M Balmaseda, ECMWF, Reading, UK L Bertino, NERSC, Bergen, Norway P Brasseur, LEGI, Grenoble, France G Brassington, Bureau of Meteorology, Melbourne, Australia J Cummings, NRL, Monterey, USA Y Fujii, JMA/MRI, Tsukuba, Japan DJ Lea, Met Office, Exeter, UK J-M Lellouche, Mercator Ocean, Toulouse, France K Mogensen, ECMWF, Reading, UK P Oke, CSIRO, Hobart, Austral...

At ISPEC ’12, Samelin et al. show that the redactable signature scheme introduced at VLDB ’08 by Kundu and Bertino does not always preserve the structural integrity of the tree signed. In particular, they show how redaction of non-leaves promotes descendants and allows a third party to add new edges to the signed tree. This alters the semantic meaning of the tree and is not acceptable in certai...