We report an isocratic "high-performance" liquid-chromatographic (HPLC) procedure for measurement of five orally administered cephalosporins (cefixime, cefaclor, cefadroxil, cephalexin, and cephradine) in 0.1 mL of human serum. Serum protein is precipitated with acetonitrile, the sample is centrifuged, and the supernate is evaporated under nitrogen. The residue is reconstituted in 0.1 mL of mob...

OBJECTIVE To assess the efficacy of gatifloxacin versus cefixime in the treatment of uncomplicated culture positive enteric fever. DESIGN A randomized, open-label, active control trial with two parallel arms. SETTING Emergency Room and Outpatient Clinics in Patan Hospital, Lagankhel, Lalitpur, Nepal. PARTICIPANTS Patients with clinically diagnosed uncomplicated enteric fever meeting the i...

BACKGROUND Neisseria gonorrhoeae (NG) infection is a serious public health problem. The third-generation extended-spectrum cephalosporins (ESCs) have been used as the first-line treatment for NG infection for almost three decades. However, in recent years, treatment failures with the oral third-generation ESCs have been reported worldwide. This study aimed to estimate worldwide susceptibility r...

OBJECTIVES There is a need for new or alternative antimicrobial agents for the treatment of gonorrhoea as antimicrobial resistance emerges to current therapies. The aim was to investigate the activity of ertapenem against isolates of Neisseria gonorrhoeae with decreased susceptibility to cefixime. METHODS A panel of 52 clinical isolates and 10 control strains of N. gonorrhoeae were selected t...

BACKGROUND Gonorrhoea is one of the most common bacterial sexually transmitted infections in England. Over 41,000 cases were recorded in 2015, more than half of which occurred in men who have sex with men (MSM). As the bacterium has developed resistance to each first-line antibiotic in turn, we need an improved understanding of fitness benefits and costs of antibiotic resistance to inform contr...

A green and convenient method for the synthesis of 2-amino-3-phenylsulphonyl-4H-chromenes by a one-pot three-component condensation of 1-naphthol, phenylsulphonylacetonitrile and aromatic aldehydes under solvent-free conditions using potassium phosphate tribasic trihydrate as an efficient catalyst is described. The reaction of 1-naphthol with phenylsulphonylacetonitrile and various aromatic...

BACKGROUND Initial antibiotic therapy in upper and lower respiratory tract infections is usually empirical. However, the decreasing susceptibility of respiratory pathogens to antibacterials have raised concerns about the decreasing efficacy of currently available antibiotics. OBJECTIVE This study was conducted to compare the efficacy and safety of cefixime and ciprofloxacin in the empirical t...

OBJECTIVES Antimicrobial resistance in Neisseria gonorrhoeae is an increasing problem worldwide and combinations of antimicrobial agents have been recommended to delay the onset of treatment failures. The objective of this study was to obtain in vitro data on the activity of current (ceftriaxone or cefixime plus azithromycin) and alternative (gentamicin plus azithromycin) regimens. METHODS A ...

The aim of the present study was to develop drug immunoconjugates (DIC) of antibiotics like Ampicillin trihydrate, Amoxycillin trihydrate, Streptomycin sulphate, Rifampicin and doxorubicin with Anti-D serum using different coupling methods. Protein content in AntiD serum was estimated by Lowry’s method. The conjugates were purified from unbound drug by dialysis. The drugs to antibody ratios wer...

The aim of this research was to evaluate solid state interaction between amoxicillin trihydrate and potassium clavulanate. The interaction was observed by Differential Scanning Calorimeter (DSC), X-Ray Powder Diffractometer (XRPD), Fourier Transforms Infra Red (FTIR) and Scanning Electron Microscope (SEM). Mixtures of amoxicillin trihydrate and potassium clavulanate were prepared in molar ratio...