نتایج جستجو برای: cdna synthesis and real
تعداد نتایج: 16967463 فیلتر نتایج به سال:
objective(s): ghrelin is a peptide hormone that has been shown to have numerous central and peripheral effects. the central effects including gh secretion, food intake, and energy homeostasis are partly mediated by kiss1- kissr signaling pathway. ghrelin and its receptor are also expressed in the pancreatic islets. ghrelin is one of the key metabolic factors controlling insulin secretion from t...
introduction ventilator-associated pneumonia (vap) is one of the most serious and prevalence of complication among patients in the intensive care unit nosocomial infections, which are often recognized after detecting symptoms. to date, there has been no proper clinical and diagnostic marker for early detection of this disease. in this study, two hcn4 and adam8 genes in patients with vap wer...
Background: Chromosome abnormalities are associated with poor morphology and development in human preimplantation embryos, all together lead to poor outcomes. This study aimed to explore altered expression of DNA damage pathways in “poor morphological and development embryos with sever aneuploidies”. Materials and Methods: Surplus day-4 embryos of PGD cases were pooled in two groups: Poor progn...
Real-time reverse transcription polymerase chain reaction (PCR) is the gold standard for expression analysis. Designed to improve reproducibility and sensitivity, commercial kits are commonly used for the critical step of cDNA synthesis. The present study was designed to determine the impact of these kits. mRNA from mouse brains were pooled to create serial dilutions ranging from 0.0625 μg to 2...
background: micrornas (mirna) are small non-coding rnas that have a distinguished role in post- transcriptional gene expression. it’s estimated that 10-30% of human mrnas are regulated by mirnas. many mirnas profiles change during normal erythropoiesis in which some of them are stage specific.mir-155 was downregulated 200 fold in erythropoiesis. materials and methods: k562cells were grown in rp...
مقدمه: سرطان پستان یک بیماری پیچیده به دلیل چندین تغییر ژنتیکی و اپیژنتیکی است که نهایتا به تغییر فرآیند های سلولی از جمله تکثیر، آپوپتوز، آنژیوژنز (رگ زایی) و اکتساب یک فنوتیپ تهاجمی منجر میشود. BIF-1(BAX interacting factor) که یک ژن سرکوب گر تومور است، در تنظیم خمیدگی های غشای میتوکندری نقش دارد و حاوی دمین BAR است که به واسطه آن با BAX تعامل می یابد. هدف از مطالعه حاضر بررسی تفاوت بیا...
background 3-4methylenedioxymethamphetamine (mdma) is a synthetic and psychoactive drug, which is known popularly as ecstasy and has toxic effects on human organs. objectives considering the potential toxic interaction, this study was performed to quantify the expression of bax and bcl2 genes in mdma-induced hepatotoxicity on rat liver. subsequently, we evaluated pentoxifylline as a possible pr...
Effective transcript profiling in animal systems requires isolation of homogenous tissue or cells followed by faithful mRNA amplification. Linear amplification based on cDNA synthesis and in vitro transcription is reported to maintain representation of mRNA levels, however, quantitative data demonstrating this as well as a description of inherent limitations is lacking. We show that published p...
worship is submission and humitility with believe to divinity in fornt of god. in jewish religion ,worship is submission of the god inall the ordes and devosion tohim .worshio is not only so widely in islam and jew and does not include pray and fast and etc but also is every action that human does with divine intention and to content him. worship has huge and excellent rank in qoran and old te...
We recently reported that hairpin (or stem-loop) priming is better-suited than polyA tailing to generate cDNA for plant microRNA qPCR. One major limitation of this method is the need to perform individual cDNA synthesis reactions for the reference gene and test miRNAs. Here, we report a novel fusion primer that allows multiplexed hairpin cDNA synthesis of the most-commonly used reference gene, ...
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