The packaging of proteins into discrete compartments is an essential feature for cellular efficiency. Inspired by Nature, we harness virus-like assemblies as artificial nanocompartments for enzyme-catalyzed cascade reactions. Using the negative charges of nucleic acid tags, we develop a versatile strategy to promote an efficient noncovalent co-encapsulation of enzymes within a single protein ca...

The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....

in order to investigate the presence of carnation etched ring virus (cerv), samples of carnation (dianthus caryophyllus), sweet william (dianthus barbatus) and weed plants showing symptoms from greenhouses of khorasan razavi and northern khorasan provinces were collected in autumn 2009. polyclonal antiserum was used in order to detection of cerv by double- antibody sandwich-enzyme linked immuno...

Carnation (Dianthus caryophyllus L.) cv. Yellow Candy flowers were treated with ethanol solution (4%) in the absence or presence of 1 or 10 mM L-methionine, or ethanol or acetaldehyde solution (0.05%), in the absence or presence of 1 mM aminocyclopropane carboxylic acid (ACC). Spermidine in petals was measured over time in flowers treated with 4% ethanol or distilled water. Ethanol treatment si...

We found that Sweet potato feathery mottle virus (SPFMV) P1, a close homologue of Sweet potato mild mottle virus P1, did not have any silencing suppressor activity. Remodeling the Argonaute (AGO) binding domain of SPFMV P1 by the introduction of two additional WG/GW motifs converted it to a silencing suppressor with AGO binding capacity. To our knowledge, this is the first instance of the trans...

The structure of the turnip crinkle virus (TCV) coat protein and coat protein gene has been examined by cDNA cloning, nucleotide sequencing and high-resolution mRNA mapping. We have cloned a 1450-nucleotide cDNA fragment, representing the 3' end of the TCV genome, using genomic RNA polyadenylated in vitro as the reverse transcriptional template. Nucleic acid sequence analysis reveals the presen...

carnation etched ring virus (cerv) is a member of the caulimovirus genus of the caulimoviridae. the virus is the second most important virus of carnation around the world and the only dna virus among infecting viruses of carnation. the virus infected plants were collected from greenhouse in chenaran and two fragments of ~1500 bp and ~1000 bp respectively for coat protein and movement protein we...