Sucrose labeled in the fructosyl ((3)H) and glucosyl ((14)C) moieties was used to quantitate extracellular polysaccharide production and degradation by cariogenic and noncariogenic oral streptococci. All of the strains produced glucan and fructan. Streptococcus salivarius produced primarily fructan, whereas S. mutans and S. sanguis produced more glucan than fructan. The cariogenic streptococci ...

We previously established murine hybridomas producing a monoclonal antibody monospecific against three glucosyltransferases (I, SI and S) of Streptococcus mutans which contribute to dental caries formation. Here, we developed a new immunochemical technique (cross-dot system) with which individual levels of glucosyltransferases expressed by S. mutans can be evaluated. We also examined glucosyltr...

The cell-associated dextransucrase produced by sucrose-grown cells of cariogenic Streptococcus mutans K1-R is derived from soluble dextransucrase. Synthesis of insoluble dextran by soluble dextransucrase gives rise to two dextransucrase fractions bound to the insoluble polysaccharide; a reversibly bound enzyme, which can be eluted in solutions of clinical dextran, and an irreversibly bound enzy...

BACKGROUND Caries excavation is a noninvasive technique of caries removal with maximum preservation of healthy tooth structure. AIM To compare the efficacy of three different caries excavation techniques in reducing the count of cariogenic flora. MATERIALS AND METHODS Sixty healthy primary molars were selected from 26 healthy children with occlusal carious lesions without pulpal involvement...

The inhibition of acid production from dental plaque and mutans streptococci by epigallocatechin gallate (EGCg), one of the green tea catechins, was examined. The effect of EGCg solution on dental plaque pH was investigated. Subjects rinsed their mouths with 2 mg/ml EGCg solution and then, after 30-min interval, rinsed their mouths with 10% sucrose. Plaque samples were collected at appropriate ...

AIM The aims of this study were to evaluate the prevention of enamel demineralization and the shear bond strength (SBS) of orthodontic brackets bonded with fluoride and no fluoride conventional and self-etching adhesives and to analyze the characteristics of enamel near the bond area using a polarized light microscope (PLM) following demineralization and remineralization cycling (Des Re). MET...

Twelve presweetened breakfast cereals were evaluated for cariogenic potential by in vitro methods. The parameters evaluated were the levels of acid produced by a cariogenic strain of Streptococcus mutans as influenced by each cereal, the percentage of sugar content, and the salivary retention time of glucose after ingestion of each cereal with or witho~¢t milk. Cariogenicity was found to be rel...

The aim of the present study was to evaluate the cariogenicity of cows', human and infant formula milks, supplemented or not with fluoride, in rats. Sixty female Wistar rats were desalivated and infected with Streptococcus sobrinus 6715.Animals were divided into six groups: group 1, sterilised deionised distilled water (SDW; negative control); group 2, 5 % sucrose added to SDW (positive control...