Rabbit antiserum to cardiolipin, which is reactive with the polar head but not the nonpolar fatty acid moieties of cardiolipin, was used to explore the location of the polar head of cardiolipin in mitochondrial membranes. Only a few per cent of the cardiolipin in intact mitochondria from rat liver, blowfly flight muscle, Saccharomyces cerevisiae, and Neurospora and none of the cardiolipin in in...

Cardiolipin is a phospholipid found in the inner mitochondrial membrane and in bacteria, and it is associated with many physiological functions. Cardiolipin has a dimeric structure consisting of two phosphatidyl residues connected by a glycerol bridge and four acyl chains, and therefore it can carry two negative charges. The pKa values of the phosphate groups have previously been reported to di...

Membranes having an a high content of cardiolipin were isolated from an extremely halophilic archaeon Halorubrum sp. Absorbance difference spectra of detergent-solubilized plasma membranes reduced by dithionite suggested the presence of b-type cytochromes. Non-denaturing gel electrophoresis revealed only one fraction having TMPD-oxidase activity in which cardiolipin was the major lipid componen...

A highly active phospholipase D that is specific for cardiolipin was detected in the gram-negative bacterium Haemophilus parainfluenzae. Previously reported phospholipase D preparations have come exclusively from higher plants. The bacterial enzyme hydrolyzed cardiolipin to phosphatidyl glycerol and phosphatidic acid. During the incubation, phosphatidic acid disappeared. Phosphatidyl ethanolami...

The phospholipids and glycerides composition of spheroplasts of Mycobacterium smegmatis ATCC 14468 was examined. The percent total phospholipids in total lipids as well as cardiolipin were found to be higher in spheroplasts as compared to their parent forms. Increase in cardiolipin and free fatty acids content and decrease in triglycerides levels were observed during spheroplasts formation. The...

31P-NMR shows that poly(L-lysine) binding to cardiolipin, phosphatidylserine or phosphatidylglycerol does not affect the macroscopic structure or local order (in the phosphate region) of the phospholipids. In the case of cardiolipin poly(L-lysine) inhibits the ability of Ca2+ to induce the hexagonal HII phase. Alternatively, poly(L-lysine) induces the hexagonal HII phase for a fraction of the p...

The phospholipid cardiolipin mediates the functional interactions of proteins that reside within energy-conserving biological membranes. However, the molecular basis by which this lipid performs this essential cellular role is not well understood. We address this role of cardiolipin using the multisubunit mitochondrial TIM23 protein transport complex as a model system. The early stages of prote...

In previous studies, the enzymatic conversion of phosphatidylglycerol to cardiolipin (diphosphatidylglycerol) in cell-free preparations from E. coli was shown to be stimulated by the addition of CDP-dipalmitin, suggesting the participation of the cytidine coenzyme as phosphatidyl donor. The present communication, however, presents three lines of evidence supporting the following mechanism for t...

Reactive oxygen species (ROS) are considered an important factor in ischemia/reperfusion injury to cardiac myocytes. Mitochondrial respiration is an important source of ROS production and hence a potential contributor to cardiac reperfusion injury. In this study, we have examined the effect of ischemia and ischemia followed by reperfusion of rat hearts on various parameters related to mitochond...

The idea of a Cardiolipin workshop in Italy came to the meeting organizers in June 2011, during the mini‐ sabbatical of Angela Corcelli in New York City in the Laboratory of Michael Schlame. They thought to take advantage of the presence of the 54th International Conference on the Bioscience of Lipids (ICBL) at Bari in 2013 to organize the Cardiolipin workshop as a satellite event. The web page...