This work describes the development of a new biosensor for the detection of an organophosphate insecticide (Diazinon) in an aqueous medium. The method is based up on the immobilization of Candida Rugosa Lipase enzyme by adsorption on Aluminosilicate in order to incorporate it into a polymeric membrane. The electrical properties of the polymeric membrane have been characterized by electrochemica...

Kinetic resolution is one of the methods which allows obtaining enantiomerically pure compounds. In study presented herein, enantioselective biotransformations (R,S)-1-phenylethanol were performed with use various catalytic systems containing ionic liquids and n-heptane or toluene as a reaction medium, vinyl acetate isopropenyl an acetylating agent, lipases from Burkholderia cepacia Candida rug...

A significant bottleneck for the industrial application of lipases stems from their poor stability in presence commercial triglycerides. This is mainly due to inactivating effect products triglyceride oxidation (PTO), which are usually produced when oils and fats, being imported far countries, stored long periods. In this study, immobilization a lipase Candida rugosa on chitosan hydrogels has b...

Hydrophobic magnetic silica aerogel was used as a support to immobilize Candida rugosa lipase by adsorption method. Physical and chemical properties of the support and immobilized lipase were determined by Field Emission Scanning Electron Microscope (FESEM), Brunauer–Emmett–Teller (BET) analysis and Fourier Transform InfraRed (FT-IR) spectroscopy and the results showed that the lipase was s...

We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked...

Conjugated linoleic acid l-menthyl ester was hydrolyzed in water by the lipase from Candida rugosa with the addition of an organic solvent. The degree of hydrolysis (yield) greatly improved when a tertiary alcohol, such as t-butyl alcohol, was added. However, the addition of a less polar solvent, such as hexane, decreased the degree of hydrolysis, and some water-miscible solvents, such as aceto...

Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficienc...

In this work, poly(n-butylamino)(allylamino)phosphazene (PBAP) was synthesized and tethered on polypropylene microporous membrane (PPMM) with the aim of offering a biocompatible and, at the same time, moderately hydrophobic microenvironment to lipase for the first time. Lipase from Candida rugosa was used and the influence of membrane surface conditions on the activities of immobilized lipases ...