In this review, we report on the different plant growth regulators (PGRs) affecting callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from explants and micropropagation through shoot tip nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of guard against liver kidney damage, stop certain cancers, regulate blood sugar levels. They benefit digestion, ...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...

Shoot tips from 4to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week....

Regeneration, a remarkable example of developmental plasticity displayed by both plants and animals, involves successive developmental events driven in response to environmental cues. Despite decades of study on the ability of the plant tissues to regenerate a complete fertile shoot system after inductive cues, the mechanisms by which cells acquire pluripotency and subsequently regenerate compl...

A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of Canavalia gladiata (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L – 1.5mg/...

The production of kencur (Kaempferia galanga L.) with tissue culture is an alternative in plant cultivation as a provider seeds. In culture, growth regulators can affect the formation organs (organogenesis). combination NAA and TDZ was used this study to induce callus organogenesis. purpose research determine effect giving on organogenesis callus, well optimal two callus. design factorial RAL f...

The present study demonstrates an efficient protocol for in vitro mass propagation of A. curassavica through direct as well as indirect regeneration. The effect of MS and L2 media supplemented with various concentrations and combinations of growth regulators has been studied. The growth regulators used include BAP and kinetin (cytokinins) and IAA, IBA, NAA and 2, 4-D (auxins). MS media proved b...