The amino-terminal regulatory domain of cardiac troponin C (cNTnC) plays an important role as the calcium sensor for the troponin complex. Calcium binding to cNTnC results in conformational changes that trigger a cascade of events that lead to cardiac muscle contraction. The cardiac N-terminal domain of TnC consists of two EF-hand calcium binding motifs, one of which is dysfunctional in binding...

The effect of ions presence such as: Na, K, Ca, Cl, SO4 and CO3, at various initial concentrations, on the kinetics of cadmium sorption by dried activated sludge was studied at 25°C and free initial pH solution in batch conditions. The presence of these ions in solution was found to inhibit slightly the uptake of cadmium by dried activated sludge at different degrees: sodium, potassium, calcium...

A new method for the simultaneous determination of lead, cadmium and mercury ions in microwave-digested foodstuffs by reversed-phase high-performance liquid chromatography combined with on-line enrichment technique has been developed. The foodstuff samples were digested by microwave digestion. The lead, cadmium and mercury ions can be precolumn derivatized with 5,10,15,20-tetrakis(3-aminophenyl...

Batch studies of cadmium ion sorption by dried sunflower leaves were carried out to identify the main rate controlling steps in the overall uptake mechanism, using a single external mass transfer diffusion model and intraparticle mass transfer diffusion model. Many classical parameters influencing cadmium sorption mechanisms namely: initial cadmium concentration, agitation speed, initial soluti...

Cadmium (Cd) is an environmental and industrial pollutant that affects the male reproductive system. Exposure to cadmium metal is known to induce the formation of reactive oxygen species (ROS) like superoxide radical, hydroxyl ion and hydrogen peroxide. Vitamin E is a lipid phase antioxidant & Vitamin C acts as a potent water soluble antioxidant by scavenging reactive oxygen species. The purpos...

Plants, like other organisms, have adaptive mechanisms whereby they are able to respond to both nutrient deficiencies and toxicities. Phytochelatins (PCs) play an essential role in heavy-metal detoxification in plants, fungi and worms. PCs chelate heavy metals and then PCmetal complexes are translocated across the tonoplast and sequestered in vacuoles. PCs are synthesized from glutathione by th...

The gene (alk G) encoding the two-iron rubredoxin of Pseudomonas oleovorans was amplified from genomic DNA by PCR and subcloned into the expression vector pKK223-3. The vector directed the high-level production of rubredoxin in Escherichia coli. A simple three-step procedure was used to purify recombinant rubredoxin in the 1Fe form. 1Fe-rubredoxin was readily converted to the 2Fe, apoprotein an...

A Cadmium (II) ion-selective electrode (ISE) based on Thia polymeric crown ether (SPCE-Cd2+) as a membrane carrier in PVC matrix was successfully built for the determination of Cd2+ ions. Using Dimethyl phthalate (DMP) plasticizer, showed Nernstian liner response spanning range 1*10-1 to 1*10-7 M with slope (28) ±2 mV per decade charge and detection limit 1*10-9 M. The sensor has 10-second time...

The effect of ions presence such as: Na + , K + , Ca 2+ , Cl , SO4 2and CO3 2, at various initial concentrations, on the kinetics of cadmium sorption by dried sunflower leaves was studied at 25°C in batch conditions. The presence of these ions in solution was found to inhibit the uptake of cadmium by dried sunflower leaves at different degrees. Na + and K + ions have no significant effect. For ...