Ca 2 1 -activated Cl channels (Cl Ca Cs) are an important class of anion channels that are opened by increases in cytosolic [Ca 2 1 ]. Here, we examine the mechanisms of anion permeation through Cl Ca Cs from Xenopus oocytes in excised inside-out and outside-out patches. Cl Ca Cs exhibited moderate selectivity for Cl over Na: P Na / P Cl 5 0.1. The apparent affinity of Cl Ca Cs for Cl was low: ...

The groundwater quality and heavy metal (HM) contamination were evaluated in palm farms, central Saudi Arabia, using pollution indices, irrigation parameters, multivariate statistical analyses. Thirty samples collected October 2020 for major anions, cations, HMs analyses interpretation. results showed that the average concentrations of total dissolved solids (TDS), Ca+, Na+, K+, Cl?, SO42?, F? ...

The contributions of electrogenic sodium/calcium exchange current ( I NaCa), calcium-activated chloride conductance [ I Cl(Ca)], and calcium-activated nonselective cation conductance to delayed afterdepolarizations (DAD) were examined. Nonselective cation channels were absent in canine M cells, since inhibition of I NaCa and I Cl(Ca)eliminated all calcium-activated currents without abolishing c...

Proteins of the CLCA gene family including the human ClCa1 (hClCa1) have been suggested to constitute a new family of chloride channels mediating Ca(2+)-dependent Cl- currents. The present study examines the relationship between the hClCa1 protein and Ca(2+)-dependent Cl- currents using heterologous expression of hClCa1 in HEK293 and NCIH522 cell lines and whole cell recordings. By contrast to ...

a total of 148 groundwater samples were collected from bore wells from thirumanimuttar sub-basin for two seasons to identify major geochemical process activated to alter groundwater chemistry. the study revealed relationships with the lithology and anthropogenic process. groundwater in the region was dominated by na – ca – mg and cl and hco3 types. the (ca +mg) versus tz+ plot for both seas...

Calcium-activated chloride channels (Cl(Ca)) are crucial regulators of vascular tone by promoting a depolarizing influence on the resting membrane potential of vascular smooth muscle cells. Niflumic acid (NFA), a potent blocker of Cl(Ca) in vascular myocytes, was shown recently to cause inhibition and paradoxical stimulation of sustained calcium-activated chloride currents [I(Cl(Ca))] in rabbit...

The effects of CLCA protein expression on the regulation of Cl(-) conductance by intracellular Ca(2+) and cAMP have been studied previously in nonepithelial cell lines chosen for low backgrounds of endogenous Cl(-) conductance. However, CLCA proteins have been cloned from, and normally function in, differentiated epithelial cells. In this study, we examine the effects of differentiation of the ...

BACKGROUND Animal studies have shown that the Ca(2+)-activated Cl(-) current (I(Cl(Ca))) and the Na(+)/Ca(2+) exchange current (I(Na/Ca)) contribute to the transient inward current (I(ti)). I(ti) is responsible for the proarrhythmic delayed afterdepolarizations (DADs). We investigated the ionic mechanism of I(ti) and DADs in human cardiac cells. METHODS AND RESULTS Human ventricular cells wer...

In sensory neurons of the peripheral nervous system, receptor potentials can be amplified by depolarizing Cl currents. In mammalian olfactory sensory neurons (OSNs), this anion-based signal amplification results from the sequential activation of two distinct types of transduction channels: cAMP-gated Ca channels and Ca-activated Cl channels. The Cl current increases the initial receptor current...

Taurodeoxycholic acid (TDC) stimulates Cl(-) transport in adult (AD), but not weanling (WN) and newborn (NB), rabbit colonic epithelial cells (colonocytes). The present study demonstrates that stimuli like neurotensin (NT) are also age specific and identifies the age-dependent signaling step. Bile acid actions are segment and bile acid specific. Thus although TDC and taurochenodeoxycholate stim...