This study was designed to confirm the protective effect of Schisandrae Fructus, which are the dried fruits of Schisandra chinensis (Turcz.) Baill, against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms in C2C12 myoblasts. Preincubating C2C12 cells with a Schisandrae Fructus ethanol extract (SFEE) significantly attenuated hydrogen peroxide (H2O2)-induced inh...

BACKGROUND Substituting galactose for glucose in cell culture media has been suggested to enhance mitochondrial metabolism in a variety of cell lines. We studied the effects of carbohydrate availability on growth, differentiation and metabolism of C2C12 myoblasts and myotubes. METHODOLOGY/PRINCIPAL FINDINGS We measured growth rates, ability to differentiate, citrate synthase and respiratory c...

Facioscapulohumeral muscular dystrophy (FSHD) is linked to the deletion of the D4Z4 arrays at chromosome 4q35. Recent studies suggested that aberrant expression of double homeobox 4 (DUX4) from the last D4Z4 repeat causes FSHD. The aim of this study is to determine transcriptomic responses to ectopically expressed DUX4 in human and mouse cells of muscle lineage. We expression profiled human rha...

Skeletal myoblasts form stable grafts in the heart and may improve myocardial performance after infarction. The current study compared the ability of different immortalized myoblast lines, or primary myoblast isolates, to form grafts in the normal or cryoinjured heart. With a constant dose of 6 x 10(6) cells, primary rat myoblasts and mouse C2C12 myoblasts formed similarly large skeletal muscle...

Although the myocardium contains progenitor cells potentially capable of regenerating tissue upon lethal ischaemic injury, their actual role in post-infarction heart healing is negligible. Therefore, transplantation of extra-cardiac stem cells is a promising therapeutic approach for post-infarction heart dysfunction. Paracrine cardiotropic factors released by the grafted cells, such as the card...

objective: adipocyte and skeletal muscle are important tissues which contribute the development and progression of metabolic disorder. insulin has a major regulatory function on glucose metabolism in these tissues by redistributing glucose transporter (glut4) from intracellular vesicles to the cell surface. today, due to the side effects of chemical medications attendance to herbal medicines is...

objective: micro-rnas (mirnas) are a class of posttranscriptional regulators that play crucial roles in various biological processes. emerging evidence suggests a direct link between mirnas and development of several diseases including type 2 diabetes (t2d). in this study, we aimed to investigate the effect of predicted mirna and target genes on insulin resistance. materials and methods: this e...