Introduction Caseins cross-reactivity rises up to 90% between cow’s milk (CM) and other bovid milks. There are few studies on tolerance to other mammals milk in patients submitted to cow’s milk oral immunotherapy (CMOIT). AIMS: To determine the percentage of patients whom after CMOIT tolerate water buffalo (WB), goat and sheep’s milk proteins (MP). To describe immunologic features which can pre...

Growth hormone, a protein hormone, has a remarkable role in growth, reproduction and milk production. The present investigation was aimed at studying the genetic variation in growth hormone gene locus 4th exon, 4th intron and 5th exon in Murrah buffalo bulls by PCR-RFLP. The growth hormone gene was amplifi ed by PCR using oligonucleotide primers standardized for Bos taurus species. A 428 bp fra...

Mastitis set apart as clinical and sub clinical is a disease complex of dairy cattle, with sub clinical being the most important economically. Of late, laboratories showed interest in developing biochemical markers to diagnose sub clinical mastitis (SCM) in herds. Many workers reported noteworthy alternation of acute phase proteins (APPs) and nitric oxide, (measured as nitrate+nitrite = NOx) in...

BACKGROUND Xanthine oxidoreductase (XOR) existing in two interconvertible forms, xanthine dehydrogenase (XDH) and xanthine oxidase (XO), catabolises xanthine to uric acid that is further broken down to antioxidative agent allantoin. XOR also produces free radicals serving as second messenger and microbicidal agent. Large variation in the XO activity has been observed among various species. Both...

In order to determine the incidence of buffaloes on enteric CH4 emissions, information about animal production and farm management was analyzed and the CH4 emission factor estimated using the IPCC Tier 2 model. CH4 emission factor for the category of buffalo cattle and buffalo bulls was estimated for the period 2000-2007, in Italy, Europe and worldwide. During 2007, the CH4 emission factor for ...

Polymorphism within the promoter region of bovine thyroglobulin has been reported to be associated with milk and meat quality. In this study, we investigated the genetic variation within thyroglobulin promoter region of swamp and riverine buffaloes using PCR-SSCP technique and sequencing, and also analyzing association of polymorphism with the milk production traits. The study revealed four con...

The current study presents the application of fluorescence spectroscopy for the identification of cow and buffalo milk based on β-carotene and vitamin-A which is of prime importance from the nutritional point of view. All samples were collected from healthy animals of different breeds at the time of lactation in the vicinity of Islamabad, Pakistan. Cow and buffalo milk shows differences at fluo...

Genetic and Economic Evaluation of Progeny Testing Selection Programs with Different Levels Artificial Insemination Milk Recording for Breeding Khuzestani Buffalo

The present investigation was aimed at studying 1.26 kb αS2-casein promoter gene in αS2casein gene locus between the three buffalo breeds. αS2 -casein promoter gene was amplifi ed by PCR using oligonucleotide primers standardized for Bos taurus species. The sizes of the amplifi cation products were similar. PCR amplicons are subjected to restriction digestion by the enzyme Eco RV, only an intac...

Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcription-polymerase chain reaction. The protein was then subjected to molecular characterization using bioinformatic methods, and the...