Background: Several studies have recently investigated the role of leptin, the polypeptide hormone secreted primarily by white adipose tissue, in the reproduction of rodents, humans, and domestic animals. Materials and Methods: To investigate the in vitro effect of leptin on buffalo semen quality in equilibrated and frozen thawed semen, five healthy buffalo bulls (5 ejaculates from each bull) w...

The present study aims to determine the effect of non-genetic factors on semen production traits Murrah buffalo bulls maintained in an organized station. data collected from 75 during period 1997 2017 were used analyse effects using least-squares analyses under General Linear Model (GLM). overall means for age at first collection (AFSC), freezing (AFSF), last (ALSC), (ALSF), (SPP), frozen (FSPP...

Aim: The present study was carried out to examine the relationships among frozen-thawed semen fertility, physical parameters, seminal quality, and testosterone concentration in Murrah buffalo bulls. Materials and Methods: A total of 30 breeding Murrah buffalo bulls (either progeny tested or under progeny testing program) were randomly selected from two government bull farms in Punjab. None of t...

Seminal plasma is complex fluid containing a wide variety of both organic and inorganic components, among which proteins are an important part of the high-molecular-weight substances. The protein composition of mammalian seminal plasma varies in species and has important effect on sperm functions such as sperm motility (Henricks et al., 1998), viability (Brandon et al., 1999) and freezability (...

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...

The present study was conducted to determine the effect of seasonal and climatic variations on semen characteristics of Nili-Ravi buffalo breeding bulls in Sahiwal and Bahawalpur districts of Punjab, Pakistan. For this purpose, 14 adult NiliRavi buffalo breeding bulls (n=7 from each of Semen Production Unit (SPU) Qadirabad, Sahiwal and SPU Karaniwala, Bahawalpur), were used. Semen samples were ...

Background: Artificial insemination is a valuable tool in genetic improvement programs and widely used breeding technique in farm animals. Semen processing and cryopreservation causes considerable damage to sperm DNA, motility apparatus, plasma membrane, acrosomal cap and thus, reduced fertility. Selenium is an integral part of glutathione peroxidase (GSH-Px), an enzyme which protects cell inte...

The presence of bovine tuberculosis (Mycobacterium bovis) in the Kruger National Park (KNP) was determined for the first time in 1990. It was diagnosed in an African buffalo (Syncerus caffer) bull, which was found recumbent and in an emaciated and moribund state near the south-western boundary fence. This prompted an investigation into the bovine tuberculosis (BTB) status of the KNP, with empha...