BACKGROUND Little is currently known about Brucella evolution within the host during infection. The current study is the first to employ fine-scale genotyping on an isolate collection derived from a Brucella canis outbreak. Eight isolates of B. canis, cultured from different tissues of three dogs (female, stud dog, puppy of another female) from a single kennel over three months were genetically...

Etiology and Epidemiology Canine brucellosis is caused by Brucella canis (B. canis), a rough, small, gram-negative, intracellular bacterium. Other Brucella species, i.e., B. abortus, B. suis, have occasionally caused canine infections but they are not discussed here. The canine brucella was first recognized in 1966 as a cause of abortions and reproductive failures and it has since been recogniz...

Brucella species are responsible for brucellosis, a worldwide zoonotic disease causing abortion in domestic animals and Malta fever in humans. Based on host preference, the genus is divided into six species. Brucella abortus, B. melitensis, and B. suis are pathogenic to humans, whereas B. ovis and B. neotomae are nonpathogenic to humans and B. canis human infections are rare. Limited genome div...

We have developed a combinatorial polymerase chain reaction (PCR) procedure to identify four major species of the genus Brucella simultaneously. Four pairs of primers targeting the genes encoding a cell surface protein (BCSP31) and outer membrane proteins (omp2b, omp2a and omp31) were prepared. PCR using these primers gave rise to specific patterns of amplification for each Brucella spp. examin...

Background: Brucella sp. are the causative agents of brucellosis, an infectious disease that affects various species of animals and can be transmitted to humans through direct contact with infected animals, indirectly by the ingestion of rawmilk products, and during the handling of strains or infected material in the laboratory. Being a zoonosis, the detection of Brucella species in animals is ...

The genus Brucella contains alpha-Proteobacteria adapted to intracellular life within cells of a variety of mammals. Controversy has arisen concerning Brucella internal taxonomy, and it has been proposed that the DNA-DNA hybridization-based genomospecies concept be applied to the genus. According to this view, only one species, Brucella melitensis, should be recognized, and the classical specie...

Bacteriological investigation of canine brucellosis in Mexico City revealed a high rate (11.8%) of Brucella canis infection in a sampling of 59 stray dogs. When conservative criteria were employed in the interpretation of serological test results, there was general agreement between the serological and bacteriological findings; however, some animals with localized male genital tract infections ...

Brucellosis is an important bacterial zoonosis in many developing countries including India. It is primarily a disease of reproductive system of sexually matured animals with concomitant loss in productivity of affected animals. The disease is caused by Gram-negative organisms of the genus Brucella that includes six species. Rodriguez et al. (1992) classified brucellae primarily on the basis of...