Expression of the virB operon, encoding the type IV secretion system required for Brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic pH values. To analyze the production of VirB proteins, polyclonal antisera against B. suis VirB5 and VirB8 were generated. Western blot analysis revealed that VirB5 and VirB8 were det...

Brucella abortus and Brucella melitensis have surface lipopolysaccharides and polysaccharides carrying B. melitensis-type (M) and B. abortus-type (A) epitopes as well as common (C) epitopes present in all smooth Brucella biotypes. Crude lipopolysaccharides, hydrolytic O polysaccharides, and native hapten polysaccharides of MC or AC specificity were evaluated in indirect enzyme-linked immunosorb...

A method of cultivating Brucella abortus S19 culture in bioreactor was attempted using three different media. Culture conditions in bioreactor were optimized by varying agitation and aeration parameters. Varying the aeration ranging from 0.5 vvm to 0.8 vvm and agitation rate ranging from 250 rpm to 400 rpm during bacterial growth was found to yield highest viable count within 48 hours of cultur...

Cellular immune responses are influential for protection against intracellular bacteria such as brucellae. Therefore, identification of Brucella abortus antigens that activate primed bovine lymphocytes is fundamental for discerning the breadth of cellular response in bovine brucellosis. Potentially antigenic components of B. abortus S19 were isolated by sodium dodecyl sulfate-polyacrylamide gel...

BACKGROUND Human brucellosis is a preventable zoonoses that may become persistent, causing, if left untreated, severe localized disease. Occupational exposure to infected animals or animal products and consumption of fresh contaminated dairy are main risk factors. METHODS One hundred farmworkers employed at two cattle farms one in Khartoum North and one in Omdurman were screened for the prese...

Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understand this host-pathogen interplay. Here, we evaluated the role of the Brucella me...

BACKGROUND The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. OBJECTIVE This study was aimed to evaluate the protection of recombinant Human Serum Albumin (HAS)-L7/L12 fusion protein in Balb/c mice. METHODS The amplified L7/L12 gene was cloned in pYHSA5 vector, pYHSA5-L7/L12 construct was tr...

Background: To prevent the losses caused by brucellosis, development of diagnostic tools for differentiating vaccinated cattle from infected one is priority in eradication disease. The present study was undertaken differential identification Brucella abortus vaccine strain S19 EryC PCR. Methods: In this study, 1145 clinical samples unvaccinated and buffaloes (200 blood samples, 710 sera, 190 va...

Background: Vaccination is the most fundamental strategy in control and eradication of brucellosis. Several vaccination programs with different vaccines have been carried out many countries which brucellosis continues to be a problem livestock. One recommended against cattle live Brucella abortus S19 vaccine. The aim this study evaluate results field safety efficacy trials for conjunctival vacc...