A study was carried out to determine the effect of enzyme concentration, temperature and incubation time of bromelain on nitrogen content (NC) and degree of hydrolysis (DH) of hydrolysate from cockle (Anadara granosa) meat wash water. Protein precipitation of cockle meat wash water was conducted at pH 4. The precipitate was then hydrolyzed using bromelain at concentrations of 0.5, 1.5 and 2.5 %...

The degree of hydrolysis and antioxidant activity protein hydrolysates from fresh cow’s milk whey obtained by the action proteolytic enzymes papain, bromelain chymosin were compared. lowest in was reported for sample MP1 (10 min reaction time, treatment with 0.1 mg/ml papain), highest percentage at hydrolysate MB12 (at 60 1.0 bromelain). For samples WC1 μl/ml chymosin), lowest. achieved WP12 us...

Recently, it has emerged that extracellular proteases have specific regulatory roles in modulating immune responses. Proteases may act as signaling molecules to activate the Raf-1/extracellular regulated kinase (ERK)-2 pathway to participate in mitogenesis, apoptosis, and cytokine production. Most reports on the role of protease-mediated cell signaling, however, focus on their stimulatory effec...

BACKGROUND Periodontitis is an inflammatory disease resulting in the destruction of periodontal tissues. Various treatment modalities have been tried in the form of mechanical therapy and surgical therapy. Antimicrobial agents have been used as a monotherapy and as an adjunct with mechanical debridement. Various plant extracts have been used as antibacterial agents. Pineapple extract (bromelian...

OBJECTIVE The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope. MATERIALS AND METHODS A total of 60 extracted human upper premolars were selected to be given standardized buccal and...

BACKGROUND AND OBJECTIVE Bromelain is a proteolytic enzyme, particularly effective in the treatment of soft tissue inflammations and traumas, in localized inflammations, especially in presence of hydropsy and also in postoperative tissue reactions. The aim of the present study is to assess the efficacy of bromelain in controlling the edema and its related pain in the inflamed area after upper t...

Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase...

Background Allergies represent a socioeconomic problem that has affected increasing world population. A range of respiratory allergens are themselves proteases or are associated with proteolytic activity. Bromelain is a mixture of cysteine proteases present in the leaves and fruit Ananas comosus, with several pharmacological applications and is widely used in the food industry. Cases of occupat...

This review focused on the usage of bromelain in numerous applications, as well most recent literature chemistry, extraction, and purification from pineapple (Ananas comosus). Bromelain is a crude, aqueous extract obtained stems fruits pineapple, leading edible member family Bromeliaceae. contains mixture different thiol endopeptidases phosphatases, glucosidases, peroxidases, cellulases, & ...

1. N-Benzoyl-L-serine methyl ester was synthesized and evaluated as a substrate for bromelain (EC 3.4.22.4) and for papain (EC 3.4.22.2). 2. For the bromelain-catalysed hydrolysis at pH 7.0, plots of [SO]/vi (initial substrate concn./initial velocity) versus [SO] are markedly curved, concave downwards. 3. Analysis by lattice nomography of a modifier kinetic mechanism in which the modifier is su...