A method for the determination of fatty acids in vegetable oils by capillary electrophoresis with indirect UV-vis detection has been developed. The separation of fatty acids was optimized in terms of Brij surfactant nature and concentration and organic modifier (2-propanol) percentage. The optimal background electrolyte consisted of 10 mM p-hydroxybenzoate, 5 mM Tris at pH 8.8, 80 mM Brij 98, 4...

The purpose of the present study was to investigate the effect of nanoemulsions as a carrier vehicle of hydrophilic drug for transdermal delivery. The response surface methodology with a mixture design was used to evaluate the effect of ingredient levels of nanoemulsion formulations including cosurfactant (isopropyl alcohol, 20 ∼ 30%), surfactant (mixed of Brij 30 and Brij 35, 20 ∼ 30%), and di...

DNA synthesis in vitro in Brij-treated Saccharomyces cerevisiae requires the product of the CDC8 gene (Hereford, L. M. & Hartwell, L. H. (1971) Nature (London) New Biol. 234, 171-172). Extracts of wild-type A364a yeast restore DNA synthesis in Brij-treated cdc8, a mutant containing a thermolabile cdc8 gene product. This constitutes a complementation assay by which the cdc8 gene product can be m...

Reducing the amounts consumed of organic solvents while keeping good chromatographic performance has been a significant step towards greening analytical methodologies. When sodium dodecyl sulfate (SDS) and Brij-35 surfactants are combined in mobile phase, they can be used as green alternative to modifiers. Surfactants have numerous advantages, including low cost toxicity, safe environmental dis...

in this work we sought to determine whether vesicles (liposomes/niosomes) were able to enhance finasteride concentration in the dermis layer, including the pilosebaceous units (psu). such enhancement could be beneficial in the treatment of some androgen-related skin disorders. hamster flank skin was used to study  3hfinasteride permeation via vesicles and a hydroalcoholic solution (ha). the dru...

Brij Kishore, M.D., M.A.M.S., M.C.C.P., F.I.C.A., F.R.S.T.M., Reader, Postgraduate Department of Medicine, Hazra, D.U., M.D., M.A.M.S., Reader in Medicine, Sachan, A.S., M.D., D.T.C.D., Reader in Tuberculosis, Agrawal, B.M., M.D., Path., Lecturer in Pathology, Bharadwaj, A.K., Ashok Kumar and Mehrotra, M.M.N., Research Scholars, Postgraduate Department of Medicine, S.N. Medical College, Agra, I...

Purified prostatic acid phosphatase catalyzes the hydrolysis of thymolphthalein monophosphate 10-fold faster if an optimal concentration of Brij 35 (a wetting agent) or protein (bovine serum albumin or human serum proteins) is present. Results of gel filtration, dialysis, and sucrose density-gradient centrifugation analysis suggest that the substrate must combine with detergent or protein befor...