Salt stress limits plant growth and crop productivity and is an increasing threat to agriculture worldwide. In this study, proteomic and physiological responses of Brassica napus leaves under salt stress were investigated. Seedlings under salt treatment showed growth inhibition and photosynthesis reduction. A comparative proteomic analysis of seedling leaves exposed to 200 mM NaCl for 24 h, 48 ...

Crucifer-feeding flea beetles, Phyllotreta spp., are chronic insect pests in Canadian prairie canola production. Multiple laboratory and field feeding bioassays were conducted to determine the susceptibility of a wide range of crucifer species, cultivars, and accessions to feeding by flea beetles with the goal of discovering sources of resistant germplasm. In 62 bioassays of 218 entries, no con...

HECATE3 (HEC3) is an important transcription factor involved in regulating the carpel and transmitting tract development in Arabidopsis thaliana. The homologous gene of HEC3 in Brassica napus (designed as BnHEC3) was obtained by in silico cloning and rapid amplification of cDNA end (RACE) method. BnHEC3 obtained from cDNA is of 748 bp, containing an open reading frame (ORF) of 546bp and coding ...

Transcription factors and their associated DNA binding sites are key regulatory elements of cellular differentiation, development, and environmental response. New tools that predict transcriptional regulation of biological processes are valuable to researchers studying both model and emerging-model plant systems. SeqEnrich predicts transcription factor networks from co-expressed Arabidopsis or ...

DNA methylation is an important regulatory mechanism for gene expression that involved in the biological processes of development and differentiation in plants. To investigate the association of DNA methylation with heterosis in Brassica, a set of intraspecific hybrids in Brassica rapa and B. napus and interspecific hybrids between B. rapa and B. napus, together with parental lines, were used t...

A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48 residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP from rapeseed were perfectly matched to those of BnD22. Thus, WSCP may be identic...

Canola (Brassica napus L.) is one of the important sources of vegetable oils and protein-rich meals. Canola ranks third in global production of oilseed crops and fifth among economically important crop next to wheat, rice, maize, and cotton. The present work was undertaken with the aim to investigate the effect of Ethylmethyl sulphonate (EMS) on the yield contributing characters of brassica nap...

A total of 16 BnaGLN1 genes coding for cytosolic glutamine synthetase isoforms (EC 6.3.1.2.) were found in the Brassica napus genome. The total number of BnaGLN1 genes, their phylogenetic relationships, and genetic locations are in agreement with the evolutionary history of Brassica species. Two BnaGLN1.1, two BnaGLN1.2, six BnaGLN1.3, four BnaGLN1.4, and two BnaGLN1.5 genes were found and name...