A seroprevalence study of bovine immunodeficiency virus (BIV) was undertaken on 1,541 serum samples from Holstein cattle from 23 herds, located in different geographical regions of Poland. The analysis was performed using ELISA, with recombinant Gag protein of BIV as antigen. The average BIV prevalence was 4.9% in individual cattle, while the percentage of herds harboring at least one seroposit...

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to...

Bovine leukemia and equine infectious anemia are registered in most regions of Ukraine many countries worldwide. This is accompanied by economic losses reduced quality livestock products, which determines the relevance study retroviral infections, their diagnosis, pathogenesis. The goal our research was to find improve methodological foundations development pathological process for a more in-de...

Addition of supramitogenic doses of phytohemagglutinin (PHA) to short-term cultures of neoplastic or nonneoplastic lymphocytes infected with bovine leukemia virus increased the synthesis of the major core virion antigen (p25) by 5- to 10-fold. Such stimulation was not due to the mitogenic effect of PHA or to a generalized increase in cellular RNA or protein synthesis but rather to enhanced tran...

Bovine buffy coat cells infected with the bovine leukemia virus (BLV) induce syncytia formation in human diploid embryonic lung cells as well as in monolayer cell cultures of bovine, simian, ovine, bat, and caprine origin, but not in mouse fibroblast cells, normall rat kidney cells, or RSV-transformed rat cells. Syncytia were not observed in diploid embryonic lung cells inoculated with bovine b...

Bovine leukemia virus (BLV), a deltaretrovirus, causes B-cell leukemia/lymphoma in cattle and is prevalent in herds globally. A previous finding of antibodies against BLV in humans led us to examine the possibility of human infection with BLV. We focused on breast tissue because, in cattle, BLV DNA and protein have been found to be more abundant in mammary epithelium than in lymphocytes. In hum...

Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product wit...

Human immunodeficiency virus type 1 (HIV-1) is a more complex retrovirus, coding for several accessory proteins in addition to the structural proteins (Gag, Pol, and Env) that are found in all retroviruses. More complex retroviruses have not been isolated from birds, and simpler retroviruses have not been isolated from humans. However, the proviruses of many endogenous simpler retroviruses are ...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...