A cross-sectional study on the epidemiological situation of bovine brucellosis was carried out in state Tocantins to evaluate effectiveness its vaccination program. The divided into five regions, and a predetermined number farms randomly selected each one. Females aged 24 months or older were farm diagnosed with by serial serology (AAT 2-ME). total 6,846 animals from 756 examined. prevalence se...

Agrobacterium, Sinorhizobium, and Ochrobactrum are genera closely related to Brucella but, in contrast to the latter, are not pathogenic for humans and animals. We studied by an indirect enzyme-linked immunosorbent assay (ELISA) the reactivities of brucellosis sera against cytosolic (CYT) and membrane (MA) antigens from these nonpathogenic bacteria, and we evaluated the potential usefulness of ...

Background: Porcine brucellosis is a contagious and emerging zoonosis but neglected in most of the endemic countries including India. The disease pigs rarely reported due to non-availability diagnostics or major focus on bovine brucellosis. Hence, necessity was felt diagnose porcine by Monoclonal antibody based ELISA, RBPT STATS for detection anti-Brucella antibodies record spatial seroprevalen...

BACKGROUND Bovine tuberculosis (BTB) and bovine brucellosis are two important milk-borne zoonoses that have been shown to be prevalent to various degrees in Ethiopian cattle. The study was carried out in four Woredas (districts) around Asella town, Arsi Zone between October 2011 and March 2012 and included 318 small-holders in 13 dairy cooperatives that marketed the delivered milk. The aims of ...

In this study, sLPS antigen coated latex bead was prepared and their evaluation was done along with RBPT, commonly employed for the serodiagnosis of brucellosis. sLPS was extracted from Brucella abortus S99 and coated on polystyrene latex beads in a phosphate buffer saline coating buffer. A total of 200 samples were collected from unorganized dairy farm and tested with RBPT and sLPS based latex...

Brucellosis is a zoonotic disease transmitted to humans by animals infected with Brucella species. Brucella species are facultative intracellular bacteria consisting of aerobic gram-negative coccobacilli. Eight species of Brucella have been identified, including B. melitensis, B. abor-tus, B. suis, B. canis, B. ovis, B. neotomae, B. ceti, and B. pinnipe-dialis. 3 The three major pathogens causi...

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react...

Recurrent spillover of Brucella abortus from wildlife reservoirs to domestic cattle in the Greater Yellowstone Area (GYA) has prevented the United States from completely eradicating bovine brucellosis. Risks to cattle are a function of the size and location of wildlife and livestock populations, the degree and nature of spatio-temporal interactions between the various hosts, the level of diseas...

Brucellosis being a global zoonotic disease and animal health problem most of the studies has been oriented towards bovine than small ruminants. Hence the present study is conducted to identify the biotype of predominant Brucella spp. in small ruminants for defined control and eradication strategies. The study was carried out by random collection of the vaginal swab, milk, blood samples from th...