Milletia aboensis has long-standing ethnopharmacological indications for the treatment of symptoms and diseases related to menopause. This investigation examined effects its phytoestrogen-rich fraction on some predictors cardiovascular risk in an ovariectomized rat model In vitro, antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Ovariectomy used trigger menop...

PURPOSE To investigate the effect of estrogen on the expression of calcium-activated potassium (KCa) channels in an overactive bladder rat model. To this end, mRNA and protein levels of KCa channel subtypes in the bladder of ovariectomized rats were measured by reverse transcription polymerase chain reaction and western blotting, respectively. METHODS Ten-week-old female Sprague-Dawley rats w...

1.1 組織標本を使用する定量解析が抱える諸問題 組織標本の解析は定性的な所見から始まる.顕微鏡を用いた丁寧な観察によって初めて得られる検体の 大きさや分布,形態などについての詳細な情報は,全ての研究の基礎となるべき重要なものである.しか しながら,定性的なデータだけでは,統計学的な検討が難しく,結果が主観的なものになりやすい.この ため,生理的組織と病的組織の比較など,さらなる研究を行う際には,定量的なデータが必要になる.定 量解析の基本的な命題は,いかにして検体の正確な数を客観的に求めることができるか,ということであ る.組織標本に基づく定量解析は単純に思われるが,正確なデータを得ることは実は容易ではない. 最大の問題は,サンプリングバイアスである(図1).これは,標本抽出の際に母集団を代表していない 検体が選ばれるために生じる偏りのことであり,2次元的な切片を使って定量解析を行...

On the basis of atomic absorption spectrophotometry rat teeth and mandible magnesium levels in experimental postmenopausal osteoporosis and after administration of 17 βestradiol were measured. The results showed that estrogen deficiency after ovariectomy decreased the magnesium content in rat teeth and mandible and administration of 17β-estradiol influences in good way the mineral components of...

To determine the roles of estrogen and constrictor prostanoids in vasopressin (VP)-induced contraction of female rat aorta, vascular reactivity to VP was determined in thoracic aortas of intact, ovariectomized, and ovariectomized + estrogen-replaced female rats in the presence of indomethacin (Indo), NS-398, SQ-29,548, or vehicle control. The effects of estrogen on vascular reactivity to the th...

Quantitative data on lung structure are essential to set up structure-function models for assessing the functional performance of the lung or to make statistically valid comparisons in experimental morphology, physiology, or pathology. The methods of choice for microscopy-based lung morphometry are those of stereology, the science of quantitative characterization of irregular three-dimensional ...

PURPOSE To evaluate the protective effects of bortezomib (Velcade) on ischemia-reperfusion (IR) injury in the rat retina. METHODS The rats were randomized to receive treatment with saline, low-dose bortezomib (0.05 mg/kg), or high-dose bortezomib (0.2 mg/kg) before the induction of IR injury. Electroretinography (ERG) was used to assess functional changes in the retina. The expression of infl...

Objective: The purpose of this study was to compare the efficacy of two NSAIDs, meloxicam and ketoprofen in controlling post-operative pain following ovariectomy in rats. Study design: Experimental study Animals: Twenty one healthy and adult female Sprague-Dawley rats weighting approximately 200 gr. Methods: Intramuscular injection of the combination of xylazine (10 mg/kg) and ketamine (75 m...

Estrogen receptor(s) (ER), progesterone receptor(s) (PGR), androgen receptor(s) (ANR), and prolactin receptor(s) (PRLR) were measured in N-nitrosomethylurea-induced mammary tumors in intact female Sprague-Dawley rats and in rats 9 days after ovariectomy. Following ovariectomy, 12 of 15 tumors regressed to 47.7 +/- 5.5% of the original size (hormone-dependent tumors), while the remaining three h...