Plasmids of the P and W incompatibility groups were introduced into Bordetella pertussis by conjugation. Plasmid DNA isolated from B. pertussis could be reintroduced by transformation. DNA isolated from Escherichia coli could not be introduced into B. pertussis by transformation if this DNA contained HindIII restriction sites. We have demonstrated that HindIII sites are modified by B. pertussis...

Determination of S-allele combinations of sweet cherry genotypes and cultivars has importance for both growers and breeders. We determined S-allele combinations of 40 local Turkish sweet cherry genotypes using a PCR-based method. Ten different S-alleles were detected. Although the most common S-allele was S3, as also found in Western genotypes and cultivars, there were some differences in the f...

The distribution of the IncFI basic replicons among IncFIV plasmids was assessed by DNA hybridization. In addition these and 20 other plasmids from 16 incompatibility groups were screened for the presence of IncIV, an incompatibility determinant recently found on the IncFIV plasmid R124. The IncIV determinant was found commonly but not universally among the IncFIV plasmids. It was also detected...

We have mapped a region of homology within a 2.2 kilobase segment of plasmids of incompatibility group F I, WHICH IS CONTAINED IN AN EcoRI restriction enzyme fragment bearing genes for autonomous replication; The coordinates of the 2.2 kilobase segment are 46.4F-48.6F on the physical map of the F plasmid. The orgin of vegetative replication has been mapped at 42.5F, at which site F has no seque...

Analysis of nucleotide sequences that regulate the expression of self-incompatibility in flowering plants affords a direct means of examining classical hypotheses for the origin and evolution of this major feature of mating systems. Departing from the classical view of monophyly of all forms of self-incompatibility, the current paradigm for the origin of self-incompatibility postulates multiple...

Forty-one stocks from 30 Drosophila species were surveyed for Wolbachia infection using PCR technology. D. sechellia and two strains of D. auraria were found to be infected and were tested for the expression of cytoplasmic incompatibility, along with D. ananassae and D. melanogaster strains, which are already known to be infected. D. ananassae and D. melanogaster show levels of incompatibility ...

Incompatibility in the context of blood groups is due to the binding of plasma antibody with red cell antigen, thereby causing a reaction. In laboratory tests this reaction is most commonly visualized by agglutination of the red cells. In the body, an antigen–antibody reaction can occur as an adverse consequence of blood transfusion or pregnancy, resulting in accelerated red cell destruction. I...