Understanding fate of antibiotic resistant bacteria (ARB) vs. their antibiotic resistance genes (ARGs) during wastewater sludge treatment is critical in order to reduce the spread of antibiotic resistance through process optimization. Here, we spiked high concentrations of tetracycline-resistant bacteria, isolated from mesophilic (Iso M1-1-a Pseudomonas sp.) and thermophilic (Iso T10-a Bacillus...

Here we present the complete 1,424,912-bp genome sequence of Coprothermobacter proteolyticus DSM 5265, isolated from a thermophilic digester fermenting tannery wastes and cattle manure.

Waste sludge is frequently treated by anaerobic digestion to kill pathogens, generate methane gas and reduce odors so the sludge can be safely land applied. In an attempt to reduce sludge volumes and improve sludge dewatering properties, the use of thermal hydrolysis (TH), a sludge pretreatment method, has been adopted by numerous wastewater treatment plants, among them being the District of Co...

A general mathematical model that predicts the flow fields in a mixed-flow anaerobic digester was developed. In this model, the liquid manure was assumed to be a non-Newtonian fluid, and the flow governed by the continuity, momentum, and k-epsilon standard turbulence equations, and non-Newtonian power law model. The commercial computational fluid dynamics (CFD) software, Fluent, was applied to ...

The ever increasing cost of fossil fuels and its attendant pollution menace has provided the pedigree to consider alternative sources of energy. An investigation was launched into the design and construction of an Anaerobic Digester system from locally available raw materials using local technology and the production of biogas from food wastes and Human excreta generated within a University cam...

In order to solve the problem of low gas production biogas digester fermented at natural temperature in winter and possibly freezing northern cold areas, combined with solar heating system is proposed maintain normal fermentation ensure continuous efficient throughout year. Through experiment analysis system, area, can be maintained above 12.5 °C, which meet requirements digester, so it conclud...

This paper examines the impact of the seed source on methanogenic abundance and overall performance of thermophilic anaerobic digesters treating the source-sorted organic fraction of municipal solid waste (SS-OFMSW). Two digesters were started up with different seed mixes and operated for 250 days until steady-state was reached. Quantitative Polymerase Chain Reaction (QPCR) was used to determin...

An understanding of sludge rheology during anaerobic digestion can improve digester performance, particularly with regards to mixing. However, the “black box” nature of digesters limits the knowledge on sludge flow behaviour, once it enters the digester. A study on the flow properties (i.e. apparent viscosity and yield stress) as well as the yielding and restructuring behaviour of primary, seco...

An aggressive start-up strategy was used to initiate codigestion in two anaerobic, continuously mixed bench-top reactors at mesophilic (37 degrees C) and thermophilic (55 degrees C) conditions. The digesters were inoculated with mesophilic anaerobic sewage sludge and cattle manure and were fed a mixture of simulated municipal solid waste and biosolids in proportions that reflect U.S. production...

The Woodman Point Wastewater Treatment Plant (WWTP) in Western Australia has experienced two separate problems causing avoidable maintenance costs: the build-up of massive struvite (MgNH4PO4. 6H2O) scaling downstream of the anaerobic digester and the formation of hydrogen sulfide (H2S) levels in the digester gas to levels that compromised gas engine operation and caused high operating costs on ...