The head region of Hydra, the hypostome, is a key body part for developmental control and the nervous system. We herein examined genes specifically expressed in the head region of Hydra oligactis using suppression subtractive hybridization (SSH) cloning. A total of 1414 subtracted clones were sequenced and found to be derived from at least 540 different genes by BLASTN analyses. Approximately 2...

Homology search finds similar segments between two biological sequences, such as DNA or protein sequences. A significant fraction of computing power in the world is dedicated to performing such tasks. The introduction of optimal spaced seeds by Ma et al. has increased both the sensitivity and the speed of homology search and it has been adopted by many alignment programs such as BLAST. With the...

RNAs Interference plays a very important role in gene silencing. In vitro identification of miRNAs is a slow process as it is difficult to isolate them. Nucleotide sequences of miRNAs are highly conserved among the plants and, this form the key feature behind the identification of miRNAs in plant species by homology alignment. In silico identification of miRNAs from EST database is emerging as ...

The problem addressed by this paper is accurate and automatic gene finding following precise identification/annotation of exon and intron boundaries for biologically verified nucleotide sequences, using the alignment of human genomic DNA to the curated mRNA transcript. We present a detailed description of a new cDNA/DNA homology gene annotation algorithm combining the results of BLASTN search w...

ABSTRACT: Aerococcus viridans is an emerging pathogen for humans and livestock animals, mainly associated with genitourinary infections cases. Its occurrence in wild mammals has never been reported. The aim of this study was to determine the etiological agent clinical a case genital infection female African elephant (Loxodonta africana). Phylogenetic analysis antimicrobial susceptibility profil...

Objective: The objective of this study was to investigate the group/subgroup phytoplasma agent in peppers showing symptoms. Material and Methods: In study, plants collected from Iğdır province 2020 were analyzed using direct nested PCR tests, BLASTn, iphyClassifier, Mega 7, pDRAW32 programs used. Results: tests performed, approximately 1.2 kb DNA fragments specific obtained. 16S rRNA nucleotide...

Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examin...

This paper explored our hypothesis that sRNA (18 ∼ 30 bp) deep sequencing technique can be used as an efficient strategy to identify microorganisms other than viruses, such as prokaryotic and eukaryotic pathogens. In the study, the clean reads derived from the sRNA deep sequencing data of wild-caught ticks and mosquitoes were compared against the NCBI nucleotide collection (non-redundant nt dat...

In the present work, we investigated the phylogenetic position and phenotypic characteristics of eight yeast isolates collected from migratory birds on the island of Ustica, Italy. A phylogenetic analysis based on the D1/D2 region of the large-subunit rRNA gene showed that all isolates clustered as a single separate lineage within the Wickerhamomyces clade. They exhibited distinct morphological...

Exon structure is relatively well conserved among orthologs in several large clades of species (e.g. Mammalia, Diptera, Lepidoptera) across evolutionary distances of up to 80 million years. Thus, it should be straightforward to predict the exon structures in novel species based upon the known exon structures of species that have had their genomes sequenced and well assembled. Being able to pred...