Sir, Carbapenem resistance in Acinetobacter baumannii is now increasingly reported. The most prevalent mechanism of carbapenem resistance in A. baumannii corresponds to the production of acquired carbapenem-hydrolysing class D b-lactamases (CHDLs) encoded by blaOXA-23-like, blaOXA-40-like or blaOXA-58-like genes. Whereas the blaOXA-40 gene has been detected in Portugal, Spain and the USA, blaOX...

The genus Acinetobacter comprises several groups of closely related species, including the Acinetobacter calcoaceticus-Acinetobacter baumannii complex (1) and a number of hemolytic species (2). Currently, the A. calcoaceticus-A. baumannii complex comprises five validly named species, including A. calcoaceticus, A. baumannii, A. pittii, and A. nosocomialis (3); the recently named species A. seif...

background the emergence of multidrug-resistant acinetobacter baumannii complicates the therapy of the related infections. hospital isolates of a. baumannii are usually multidrug-resistant. the problem is compounded by increasing resistance to broad-spectrum antibiotics including carbapenems. objectives the aim of this study was to determine antimicrobial susceptibility patterns and distributio...

The gene encoding the carbapenemase OXA-181 (an OXA-48 variant) was identified from a Citrobacter freundii isolate coproducing NDM-1. The whole sequence of plasmid pT-OXA-181 bearing the blaOXA-181 gene was determined and revealed a 84-kb mobilizable but non-self-conjugative IncT-type plasmid. It totally differs from the 7.6-kb ColE-type and blaOXA-181-bearing plasmid recently identified in a K...

The development of carbapenem-resistant Acinetobacter species is of serious concern in the hospital settings and naturally occurring oxacillinase genes (blaOXA) have been identified in several Acinetobacter species. In this study, we report the genome sequence of A. pittii TCM178 belongs to ST950, a multidrug-resistant isolate that harbored the blaOXA-72 and blaOXA-533 genes simultaneous. The g...

and New Zealand (3–5,7). We were unable to confi rm the original source of these isolates, and continuous surveillance for carbapenemase producers in our hospital has not uncovered any blaOXA-181–positive isolates since 1996. To our knowledge, there are no reports of blaOXA-181– positive isolates in Bangladesh. However, this country borders India, which is a source of blaOXA-181–positive Entero...

An alarming increase in the resistance rates of tigecycline and colistin among carbapenemase-producing Acinetobacter baumannii recovered from a Greek hospital over a 3-year period (2011-2013) was investigated. The antimicrobial resistance profiles and carbapenemase gene content were determined for a collection of colistin- and/or tigecycline-resistant carbapenemase-producing A. baumannii isolat...

Using Nanopore sequencing, we describe here the circular genome of an Escherichia coli sequence type 410 (ST410) strain with five closed plasmids. A large 111-kb incompatibility group F (IncF) plasmid harbored blaNDM-5 and 16 other resistance genes. A 51-kb IncX3 plasmid carried QnrS1 and blaOXA-181E. coli isolates with both blaNDM-5 and blaOXA-181 carbapenemases are rare.

Acinetobacter species are a major cause of hospital-acquired infections, especially in intensive care units (ICUs) (1). This, in part, is due to the bacteria’s ability to survive long-term on hospital environment surfaces and their ability to acquire antibiotic resistance genes (2). One of the Acinetobacter species most often associated with high morbidity and mortality is A. baumannii (1). How...

OBJECTIVES Bacteria of the genus Acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. Species identification is difficult and there is a need for simple molecular methods to differentiate between the species. Naturally occurring oxacillinase genes (blaOXA) have been identified in several Acinetobacter species and their detection by PCR ...