AIMS In this study, we examined the biofilm formation of 75 Salmonella enterica serovar Typhimurium (Salm. Typhimurium) human clinical isolates and the effect of subinhibitory concentrations (sub-MICs) of gentamicin, ciprofloxacin and cefotaxime on biofilm formation and exopolysaccharides (EPS) production. METHODS AND RESULTS Quantification of biofilm formation and EPS production were carried...

background & objectives:  methicillin resistance staphylococcus aureus (mrsa) is one of the most important pathogens that causes several nosocomial and community infections. adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. the aims of this study were to determine presence oficaabcd genes and relation to the biofilm formation in of mrsa isolates...

Histophilus somni is an opportunistic pathogen causing respiratory, genitourinary and generalized infections in cattle. An important virulence factor is its ability to produce a biofilm. The aim of this work was to confirm that H. somni Hsp60 (Gro-EL) is a constituent of the biofilm produced by this bacterium in vitro and to check whether or not the presence of a specific antibody within the cu...

conclusions: high percentage of hemolysins, biofilm and beta lactamase production by isolated staphylococci, suggests an important role of these virulence factors in the pathogenesis of isolated staphylococci from dermatitis lesions. the s. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics. only ciprofloxacin was found to be active against all is...

Biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis were determined by the production of protease and lipase. Inhibition of lipolytic and proteolytic activity will lead to control of virulence factors produced by S. aureus and S. epidermidis. Antibiofilm activity of isolated compound Vancomycin against S. aureus and S. epidermidis showed significant results. The effect of ...

Biofilms are defined as bacterial communities surrounded by a structured extracellular polymeric matrix which is adhered to biotic or abiotic surfaces [1]. Biofilm formation develops in two steps: firstly, a primary attachment occurs onto polymeric surfaces, mediated partly by cell-wall associated adhesins; secondly, cell-cell proliferation forms a multilayered biofilm, mediated by production o...

A group of biofilm-producing bacteria isolated from patients with urinary tract infections was evaluated, identifying the main factors contributing to biofilm formation. Among the 156 isolates, 58 (37.2%) were biofilm producers. The bacterial species (P<0.001), together with patient's gender (P = 0.022), were the factors with the highest influence for biofilm production. There was also a strong...

Cystic fibrosis (CF) patients typically suffer of persistent and recurrent lung infections caused by Pseudomonas aeruginosa that many times possess ability for the biofilm production. Here, biofilm production among P. aeruginosa isolates recovered from sputum of CF and non-CF patients was evaluated. Most isolates were biofilm-producing independently of the patient's condition.

Previously we discovered that OmpA of Escherichia coli increases biofilm formation on polystyrene surfaces (González Barrios et al., Biotechnol Bioeng, 93:188-200, 2006a). Here we show OmpA influences biofilm formation differently on hydrophobic and hydrophilic surfaces since it represses cellulose production which is hydrophilic. OmpA increased biofilm formation on polystyrene, polypropylene, ...

Biofilm production is an important mechanism for bacterial survival and its occurrence together with antimicrobial resistance represents a challenge for clinical management. Here, we evaluated the ability for biofilm production among P. aeruginosa isolates from patients with or without cystic fibrosis (CF) using two distinct media, besides determining the antimicrobial susceptibility profile of...