conclusions our results indicated that an enormous proportion of k. pneumoniae isolated from sputum and surgical-wound swabs produced fully established biofilms. it is reasonable to assume the existence of a relationship between the site of infection and the formation of biofilm. a high level of genetic diversity among the k. pneumoniae strains was observed. results the distributions of the cli...

A general multidimensional, multispecies, heterogenous biofilm model is developed using the balance equations. Multidimensional effects are studied by taking limiting scenarios towards lower dimensional analogs, as well as studying the effects of changing biofilm surface geometries. Error maps are developed suggesting when single-dimensional models give an accurate representation of biofilm gro...

Pseudomonas aeruginosa and Staphylococcus aureus are ubiquitous pathogens often found together in polymicrobial, biofilm-associated infections. This study is the first to use laser ablation electrospray ionization mass spectrometry (LAESI-MS) to rapidly study bacteria within a mixed biofilm. Fast, direct, non-invasive LAESI-MS analysis of biofilm could significantly accelerate biofilm studies a...

Nowadays silver nanoparticles (AgNPs) are used as antimicrobial due to its well known physical, chemical, and biological properties. A large collection of bacterial cells adhering to a surface is called bacterial biofilm. Exposure to silver nano particles (AgNPs) may prevent colonization of new bacteria onto the biofilm. In the present work, we have investigated whether the biofilm format...

Objective(s):The inhibitory effects of iron chelators, and FeCl3 chelation on biofilm formation and swarming motility were investigated against an opportunistic human pathogen Pseudomonas aeruginosa. Materials and Methods:The inhibitory activity of 2,2’-bipyridyl, lipoic acid, kojic acid and picolinic acidonbiofilm formation of P. aeruginosa strain PAO1 and three clinical isolates (P. aeruginos...

We evaluate a method for biofilm disinfection by raising biofilm temperature using the photothermal effect of a gold nanorod cluster. Gold nanorods (GNRs) are capable of generating enough heat to lyse bacteria by heating biofilm via laser irradiation. To test this, GNRs are synthesized using wet chemistry and a single GNR cluster is fabricated using photo-lithography technique. The GNR cluster ...

Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the culture extract of a taxonomically novel species of Paenibacillus strain 139SI for its inhibitory ...

An understanding of the regulatory mechanisms that drive Staphylococcus aureus biofilm formation may lead to the development of an effective strategy to control the increasing number of refractory clinical infections it causes. The present study examined the effects of the antimicrobial agent human β‑defensin 3 (hBD‑3) and the antibiotics vancomycin and clindamycin on the expression of the S. a...

Biofilm formation by the opportunistic fungal pathogen Candida albicans is a complex process with significant consequences for human health: it contributes to implanted medical device-associated infections. Recent advances in gene expression profiling and genetic analysis have begun to clarify the mechanisms that govern C. albicans biofilm development and acquisition of unique biofilm phenotype...