Recent metabarcoding work in European rivers, including our own studies of Mediterranean rivers Catalonia (Pérez-Burillo et al. 2020), has shown promising agreement between morphology- (LM) and DNA-based (HTS) assessments diatom periphyton for the Water Framework Directive. However, 10 out 164 Catalan sites we analysed, ecological status class was downgraded from “Good”/ “High” with LM to “Mode...