نتایج جستجو برای: bio pcr
تعداد نتایج: 233744 فیلتر نتایج به سال:
We evaluated the clinical performance of the Bio-Rad Dx CT/NG/MG assay for the detection of Chlamydia trachomatis, Mycoplasma genitalium and Neisseria gonorrhoeae in urogenital samples in comparison with the Roche COBAS® TaqMan® CT assay for C. trachomatis and an in-house TaqMan PCR assay for M. genitalium. Swab specimens were cultured for N. gonorrhoeae. In this prospective study, urogenital s...
In this work, the effects of two commercial bio-fungicides Biosubtil (Bacillus subtilis) and the bio-fertilizer Biophosphorus (Pseudomonas species), was assayed on the disease incidence, changes in the defensive enzyme activity, the total phenolic content and the expression level of some defense-related genes using real-time PCR method. Biochemical and molecular changes were measured at four ti...
The current spread of zoonotic visceral leishmaniasis (ZVL) throughout arid areas of Central Tunisia is a major public health concern. The main objective of this study is to investigate whether the development of irrigation in arid bio-geographical areas in Central Tunisia have led to the establishment of a stable cycle involving sand flies of the subgenus Larroussius and Leishmania infantum, a...
Opisujemo tridesetdvogodi{njeg bolesnika zara`enog HIV-om koji se razbolio pet mjeseci prije prijema u Kliniku s progresivnim mr{avljenjem, febrilitetom i dijarejom. Zadnja 4 dana prije prijama osjetio je ja~e bolove u trbuhu. U ~asu prijama u bolnicu bio je afebrilan, orijentiran, usporen, blijed, afoni~an, klonuo, kahekti~an i nepokretan. Abdomen je bio mekan, difuzno lagano osjetljiv na palp...
We compared the performances of various DNA extraction kits for their ability to recover Brucella abortus strain 19 inoculated into Brucella-free bovine tissues. Tissues were homogenized in a FastPrep bead homogenizer and extracted in triplicate by using one of five kits (Qiagen DNeasy, GE Illustra, Omega Bio-tek E.Z.N.A., Quanta Extracta, and IBI Science DNA Tissue kit). Whole blood was also t...
A total of 355 cows were sampled (serum, n = 315; faeces, n = 355; milk, n = 209) from dairy farms located in the Punjab state of India. Faeces and serum/milk samples were screened by acid fast staining and "indigenous ELISA," respectively. IS900 PCR was used to screen faeces and milk samples. Bio-load of MAP in dairy cows was 36.9, 15.6, 16.3, and 14.4%, using microscopy, serum ELISA, milk ELI...
Probabilistic roadmaps are an effective tool to compute the connectivity of the collision-free subset of high-dimensional robot configuration space. This paper extends them to capture the pertinent features of continuous functions over high-dimensional spaces. This extension has several possible applications, but the focus here is on computing energetically favorable motions of bio-molecules. M...
Abstract Purpose The anaerobic digestion of the organic fraction municipal solid waste (OFMSW) is a treatment biologically optimizable. analysis microbial balance within digester allows not only to describe and improve efficiency system but also foresee potential variations after biochemical interventions. This work aims highlight methanogenic population in two similar parallel digesters identi...
Polyhydroxybutyrate (PHB) is a polyhydroxy alkanote (PHA) which produces in ralstonia eutropha by three enzymes, including Beta-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase and PHB synthase. These three enzymes are encoded by phbA, phbB and phbC respectively. There is considerable interest in Polyhidroxybutirate, since it can be used as biodegradable plastic, which under optimal cond...
This introductory laboratory exercise gives first-year life science majors or nonmajors an opportunity to gain knowledge and experience in basic bioinformatics and molecular biology laboratory techniques and analysis in the context of a mock crime scene investigation. In this laboratory, students determine if a human (Lady) or dog (Kona) committed the fictional crime of scaring a cat. Students ...
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