Human serum albumin (HSA) is the most abundant protein in the blood plasma. Drug binding to HSA is crucial to study the absorption, distribution, metabolism, efficiency and bioavailability of drug molecules. In this study, isothermal titration calorimetry and molecular dynamics simulation of HSA and its complex with indometacin (IM) were performed to investigate thermodynamics parameters and th...

background: the most reliable indicator of vitamin d status is circulating concentration of 25‑hydroxycalciferol (25(oh) d) routinely determined by enzyme‑immunoassays (eia) methods. this study was performed to compare commonly used competitive protein‑binding assays (cpba)‑based eia with the gold standard, high‑pressure liquid chromatography (hplc). methods: concentrations of 25(oh) d in sera ...

background: analysis of human cancer serum has revealed the presence of high amounts of hyaluronan (hyaluronic acid, ha) when compared to human normal serum, it is well documented that ha and its receptors, known as hyaladherins (habps) are involved in matrix regulation, cell proliferation, migration and malignant tumour progression. these hyaladherins not only interact with hyaluronan at the m...

Abstract The pandemic of Coronavirus Disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). viral fusion protein, spike binds to its receptor Angiotensin-Converting Enzyme (ACE2) enter host cells. Further, the S1 subunit protein may be cleaved off virus and affect various organs. In addition tightly binding ACE2, also enhances peptidase activity ACE2 activate cel...

Abstract In December 2019, a novel respiratory tract infection, from severe acute syndrome coronavirus 2 (SARS-CoV-2), was detected in China that rapidly spread around the world. This virus possesses spike (S) glycoproteins on surface of mature virions, like other members coronaviridae. The S glycoprotein is crucial viral protein for binding, fusion, and entry into target cells. Binding recepto...