We report the complete primary and secondary structures of a metas tasis-associated M, 34,000 galactoside-binding lectin. The polypeptide sequence (264 amino acids) was derived from the nucleotide sequence of three overlapping complementary DNA clones isolated from Xgtll and XgtlO phage libraries of UV-induced murine fibrosarcomas. Striking features of the polypeptide sequence are two distinct ...

DORN1 (also known as P2K1) is a plant receptor for extracellular ATP, which belongs to a large gene family of legume-type (L-type) lectin receptor kinases. Extracellular ATP binds to DORN1 with strong affinity through its lectin domain, and the binding triggers a variety of intracellular activities in response to biotic and abiotic stresses. However, information on the tertiary structure of the...

We report the complete primary and secondary structures of a metas tasis-associated M, 34,000 galactoside-binding lectin. The polypeptide sequence (264 amino acids) was derived from the nucleotide sequence of three overlapping complementary DNA clones isolated from Xgtll and XgtlO phage libraries of UV-induced murine fibrosarcomas. Striking features of the polypeptide sequence are two distinct ...

Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted grouper, Epinephelus akaara. The gene struc...

The lima bean lectin recognizes terminal alpha-D-GalNAc groups and agglutinates human type A erythrocytes. We have cloned a portion of the gene encoding the alpha subunit of the lima bean lectin. The clone was obtained using the polymerase chain reaction and verified from a genomic clone encoding the mature protein of 253 amino acids. The deduced amino acid sequence has significant overall homo...

Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II antifreeze proteins from herring and smelt require Ca2+ for binding to ice, wher...

MicroRNAs (miRNAs) are a type of noncoding RNA participating in the post-transcriptional regulation gene expression that regulates plant responses to salt stress. Small sequencing was performed this study discover miRNAs responding stress Taxodium hybrid ‘Zhongshanshan 405’, which is tolerant salinity A total 52 were found be differentially expressed. The target genes enriched with ontology (GO...

The ferredoxin (fdx) and lectin (hgl5) promoters of Entamoeba histolytica contain the DNA sequence motif TATTCTATT (URE3). Previously we showed that mutation of the URE3 motif in the hgl5 lectin promoter results in an increase in promoter reporter activity. Mutation of this motif in the fdx promoter led to a 40-to-50% decrease in fdx promoter activity as measured by reporter gene activity and a...

A fusion between the genes encoding the marine bacterium Cobetia marina alkaline phosphatase (CmAP) and Far Eastern holothurian Apostichopus japonicus mannan-binding C-type lectin (MBL-AJ) was performed. Expression of the fusion gene in E. coli cells resulted in yield of soluble recombinant chimeric protein CmAP/MBL-AJ with the high alkaline phosphatase activity and specificity of the lectin MB...