In normal fibroblasts CDKs exist predominantly in p21/PCNA/cycl in/CDK quaternary complexes, whereas in p53-deficient cells, p21 expression is depressed and the kinases are reduced to a cyclin/CDK binary state, p21 is a universal cyclin kinase inhibitor, but we show here that p21-containing complexes exist in both catalytically active and inactive forms. This finding challenges the current view...

The purpose of the present study was to investigate the usefulness of the ternary complex with protamine and γ-polyglutamic acid (γ-PGA), which are biodegradable materials for foods and medical products, as a safe gene delivery vector. We formed cationic binary complexes (plasmid DNA (pDNA)/protamine complexes) with high transfection efficiency. The binary complex showed slight toxicity probabl...

objective(s): the aim of this work was to investigate the effect of the natural and the chemically modified form of cyclodextrins namely; β-cyclodextrin (β-cd) and hydroxypropyl-β-cyclodextrin (hp-β-cd) respectively on the solubility and dissolution rate of aripiprazole; an antipsychotic medication showing poor aqueous solubility.   materials and methods: phase solubility of aripiprazole with t...

Designer multicomponent lipoplexes have recently emerged as especially promising transfection candidates, since they are from 10 to 100 times more efficient than binary complexes usually employed for gene delivery purposes. Here, we show, for the first time, that after internalization binary complexes of lower transfection potency remain in compact perinuclear endosomes, while multicomponent sy...

the stability constants of species present in the systems ni(ii)-pyridoxamine(pym)(a) and ni(ii)-pyridoxamine(pym)(a)-imidazole containing ligands(b) [b = imidazole(him),  benzimidazole(bim), histamine(hist) and l-histidine(his)] have been determined ph-metrically using the miniquad computer program. the existence of the species niah, nia and nia2 was proven for the ni(ii)-pym(a) system, wherea...

The effects of disulfiram, its metabolite diethyldithiocarbamate and dithiodipyridine on alcohol metabolism of equine hepatic alcohol dehydrogenase (EC.1.1.1.1.) have been investigated. They were found to form enzyme-NAD(+)-inhibitor complexes which were competitive inhibitors of alcohol metabolism with dissociation constants (KEO,I) at pH 7.0 of 50 microM, 1.3 mM, and 260 microM, respectively....

Calixresorcinarenes have proved to be unique molecules for molecular recognition via hydrogen bonding, hydrophobic and ionic interactions with suitable substrates such as cations. The study of the interactions involved in the complexation of different cations with calixresorcinarenes in solvent mixtures is important for a better understanding of the mechanism of biological transport, molecular ...

Copper, cadmium, and phthalic acid (H2Lp) adsorption by ferrihydrite was examined for binary and ternary systems. In binary systems adsorption was well reproduced using the diffuse layer model (DLM), and H2Lp adsorption was analogous to that of inorganic diprotic acids in terms of the relationship between the adsorption constants and acidity constants. In ternary systems H2Lp caused both the en...

The detection of bacterial spores via dipicolinate-triggered lanthanide luminescence has been improved in terms of detection limit, stability, and susceptibility to interferents by use of lanthanide-macrocycle binary complexes. Specifically, we compared the effectiveness of Sm, Eu, Tb, and Dy complexes with the macrocycle 1,4,7,10-tetraazacyclododecane-1,7-diacetate (DO2A) to the corresponding ...

The stability constants of Fe(III) and Cr(III) with methyl cysteine and nitrilotriacetate (NTA) were determined by paper electrophoretic technique. Beside binary ternary complexes have also been studied, in which nitrilotriacetate and methyl cysteine acts as primary and secondary ligand, respectively. The stability constants of mixed ligand complexes metal (M)-nitrilotriacetate-methyl cysteine ...