Cytochrome P-450-catalyzed epoxidation of (-)-(7R,8R)-d\hydroxy-7,8-dihydrobenzo(a)pyrene to (+H7fl,8S)-dihydroxy(9S,10fl)-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene is now well recognized as the primary pathway by which benzo(a)pyrene is converted to an ultimate carcinogen. The present metabolism studies of 6-fluorobenzo(a)pyrene indicate (a) that the 6-fluoro substituent does not dramatically bl...

polycyclic aromatic hydrocarbons (pahs) are a class of complex substances that are produced during incomplete combustion of fossil fuels. pahs are known to be persistent pollutants which can remain in nature for years. in 1991 during the first persian gulf war south west part of iran was contaminated heavily. this study was undertaken to see if the pahs can be traceable in animal tissues many y...

The environment is heavily populated with polycyclic aromatic hydrocarbons (PAHs), which are dangerous to human health. Degradation and cleaning of PAH chemicals from water soil regions crucial due their chemical biological impacts persistent nature. In this study, we found that a very efficient bacterial consortium A-LOBP-19A+LOP-9 (99.62%) for benzo[a]pyrene up 1000ppm B-LOP-9 +GWP-2 (93.8%) ...

The tumorigenic activities of benzo(e)pyrene, 9,10-dihydrobenzo(e)pyrene, 9,10-epoxy-9,10,11,12-tetrahydrobenzo(e)-pyrene, the diastereomeric bay-region 9,10-dihydroxy-11,12-epoxy-9,10,11,12-tetrahydrobenzo(e)pyrenes, and the K-region benzo(e)pyrene 4,5-oxide were assessed in newborn mice, Swiss-Webster mice received a total dose of 0.7 mumol of compound divided into three i.p. injections of 0....

Human exposure to chemical carcinogens is an important etiological factor in cancer diseases. In this article, we will discuss a new approach to the development of anticarcinogenic vaccines. The main task in our research was to select a benzo[a]pyrene immunomimetic peptide considered as a hapten-specific component. For this purpose, we synthesized carcinogen-protein conjugates and prepared mono...

Batch experiments were conducted to characterize the degradation of benzo[a]pyrene, a representative high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH), by Sphingomonas yanoikuyae JAR02. Concentrations up to the solubility limit (1.2 microg l(-1)) of benzo[a]pyrene were completely removed from solution within 20 h when the bacterium was grown on salicylate. Additional experiments...

The effects of chrysotile, water-leached chrysotile, and silica on microsomal metabolism of benzo(a)pyrene in vitro were studied. Examination of benzo(a)pyrene metabolites generated by 3-methylcholanthrene-pretreated rat liver microsomes, in the presence of chrysotile fibers, revealed a reduction in the overall metabolism of the hydrocarbon. Thus, chrysotile appeared to modify the activities of...

Soil microorganisms play an important role in the degradation of PAHs and use various metabolic pathways for this process. The effect soil pH, different amendments co-cultivation fungi on activity ligninolytic enzymes was evaluated. For that purpose, three were studied: Trichoderma viride, Penicillium chrysogenum Agrocybe aegerita. Biodegradation assays with a mixture 200 ppm (fluorene, pyrene,...

The roles of two species of cytochrome P-450, the major cytochrome P-450 components of liver microsomes of phenobarbital-treated rats (PB-P-450) and 3-methylcholanthrene-treated rats (MC-P-448), were studied in the metabolism of benzo(a)pyrene in rat liver microsomes in vitro. Benzo(a)pyrene was incubated with polychlorinated biphenyl-treated rat liver microsomes, in which PB-P-450 and MC-P-448...

We propose that the cellular burden of certain carcinogens may be mitigated by P-glycoprotein (P-gp), the putative drug efflux pump. In a series of multidrug resistant human breast cancer MCF-7 cells with increasing P-gp expression we examined this hypothesis using benzo(alpha)pyrene, a widely distributed environmental and dietary carcinogen. We found that multidrug resistant cells were cross-r...