Vajner L. , J . Uhl ík , V. Konrádová, J . Zocová: The Effect of Intravenously Administered Acetylcholine on the Glycoconjugate Composition in Goblet Cells of the Tracheal Epithelium in Rabbits. Acta Vet. Brno 2000, 69: 17-23. To evaluate the influence of cholinergic stimulation on the composition of glycoconjugates in goblet cells, we studied the tracheal epithelium of rabbits 5 minutes and 20...

Human von Willebrand factor (vWF) mediates platelet adhesion and thrombus formation under high shear stress conditions; moreover, it binds and stabilizes procoagulant factor VIII in circulating blood. Quantitative and qualitative vWF abnormalities lead to a congenital bleeding disorder: von Willebrand disease (vWD). Mature vWF circulates in plasma as a series of multimers ranging in size from 4...

A mouse gene cloned in the laboratory of James and Olivia Smith at Baylor College of Medicine was found to be involved in motor function. This gene was named CRFl for Clq-Related Factor 1, and using in situ hybridization, it was found to be expressed mainly in the brainstem of adult mice. There is a gene similar to the CRFl gene in humans. By determining this gene's role in mouse development, m...

Glanzmann thrombasthenia (GT) is an inherited bleeding disorder due to either absence or dysfunction of fibrinogen binding receptors, i.e either GPIIb (GPIIβ) or GPIIIa (GPIIIα) on platelet membrane. The complete fibrinogen receptor, i.e. GPIIβGPIII which binds fibrinogen on activated platelets involves association of these two glycoproteins. Absence of either or both of these receptors can, th...

Treatment of Staphylococcus aureus infections has become increasingly challenging due to the rise of antibiotic-resistant strains. Therefore, development of antibiotic-independent treatments that supplement or provide an alternative approach to traditional therapies is greatly needed. One promising approach is the development of ‘antipathogenic’ therapies that inhibit bacterial virulence. Quoru...

Transient expression of either a or a1b subunits of the high-conductance Ca21-activated K1 (maxi-K) channel has been achieved in COS-1 cells. Expression has been studied using charybdotoxin (ChTX), a peptidyl inhibitor that binds in the pore on the a subunit. Although some properties of monoiodotyrosine-ChTX (125I-ChTX) binding to membranes derived from each type of transfected cells appear to ...

Green fluorescent protein (GFP) has been widely used as a live reporter gene in cells both in vitro and in vivo (1,4,8–10,12–14). Herpes simplex virus type-1 (HSV-1) amplicon vector has been used for gene transfer into tumor and normal quiescent neural cells in vivo and successful gene expression has been achieved (2,3,5–7,11,15). However, we and others have attempted but failed to directly obs...

Typhoid fever is still an important public health problem in many developing countries especially in tropical parts of the world, as in Indonesia. This problem opens the way for a further study with the aim of finding an alternative serological test with a high degree of reliability for the detection of typhoid fever. Given the above mentioned purpose, a study on the reliability of a laboratory...

Different autoantigens are thought to be involved in the pathogenesis of insulin-dependent diabetes mellitus, and they may account for the variation in the clinical presentation of the disease. Sera from patients with autoimmune polyendocrine syndrome type I contain autoantibodies against the 13-cell proteins glutamate decarboxylase and an unrelated 51-kDa antigen. By screening of an expression...