Rat lungs were inflated and incubated in either anionic or cationic ferritin, and alveolar and capillary basement membranes were examined by electron microscopy. Cationic ferritin bound to heparan sulfate proteoglycans on the external surface of the alveolar basement membrane, whereas cationic ferritin bound to the lamina densa of the capillary basement membranes. Anionic and cationic ferritin ...

Mice were injected intravenously with beef liver catalase (mol wt 240,000) and very small doses of horseradish peroxidase (mol wt 40,000) and the site of localization of these enzymes in the kidney was studied by ultrastructural cytochemistry. 1 min after injection, catalase was present in glomerular capillary lumina and there was minimal permeation of the basement membrane. After 5-180 min, st...

Laminin is a large basement membrane glycoprotein localized in the trophoblast, glomerular basement membrane and in the mesangial matrix of human glomeruli. It promotes the attachment of epithelial cells to basement membrane collagen. We have found that 14 sera from 52 patients with severe preeclampsia or eclampsia contain IgG and IgM antibodies which react with placental and kidney basement me...

THE problem of the splitting of the glomerular basement membrane originated with the observations of Jones (I953, I957) on the histogenesis of membranous thickening, made possible by means of a new method of staining (silver-methenamine). In his opinion this thickening was caused by the splitting of the basement membrane due to the deposition of a hyaline substance between the two layers. Jones...

Nidogens are highly conserved proteins in vertebrates and invertebrates and are found in almost all basement membranes. According to the classical hypothesis of basement membrane organization, nidogens connect the laminin and collagen IV networks, so stabilizing the basement membrane, and integrate other proteins. In mammals two nidogen proteins, nidogen-1 and nidogen-2, have been discovered. N...

Anti-glomerular basement membrane disease is characterized by autoimmunity to antigenic sites on type IV collagen of the glomerular basement membrane. The majority of patients present with rapidly progressive glomerulonephritis and alveolar hemorrhage. The occurrence of antiglomerular basement membrane disease and other types of glomerulonephritis, such as membranous nephropathy, IgA nephropath...

The basement membrane plays an important role in maintaining the structural and functional integrity of tissues. Altered basement membrane structure has been associated with severe functional impairment of the testis in several conditions, including vasectomy, autoimmune orchitis, cryptorchidism, and following x-irradiation. We have used efferent duct ligation as a model to examine seminiferous...

Studies of the pathogenesis of glomerular disease have suggested that polymorphonuclear leucocytes promote glomerular damage. The pattern of events observed in the autologous phase of nephrotoxic serum nephritis in rabbits indicates that the fixation of antibodies to glomerular basement membrane initiates the activation and binding of complement. This results in the chemotaxis of polymorphonucl...

Integrated Ocean Drilling Program Expedition 301 was part of a long term series of operations and experiments focused on quantifying hydrogeologic, lithologic, biogeochemical, and microbiological properties, processes, and linkages in basaltic crust on the eastern flank of the Juan de Fuca Ridge. This paper summarizes peer-reviewed hydrogeologic studies published since the end of Expedition 301...