In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community. Here, we developed bioreactors for producing CH₄ and hydrogen (H₂) using anaerobic groundwater collected from the deep aquifer. To generate CH₄, the anaerobic groundwater amended with organic substrates was incubated in the bioreactor. At first, H₂ was detected a...

The potential of bacterium-bound plasmin to degrade mammalian extracellular matrix and to enhance bacterial penetration through basement membrane was assessed with the adherent strain SH401-1 of Salmonella enterica serovar Typhimurium. Typhimurium SH401-1 was able to bind plasminogen and to enhance the tissue-type plasminogen activator-mediated activation of the single-chain plasminogen to the ...

nano-films consisting of an alternating sequence of positively and negatively charged polyelectrolyteshave been prepared by means of the electrostatic layer-by-layer sequential assembly technique on mildsteels. the mild steels were pretreated electrochemically to modify the mild steel surface. the modificationof the mild steel surface resulted in increasing the adhesion of the obtained nano-fil...

A bacterial strain able to use cyanide as the sole nitrogen source under alkaline conditions has been isolated. The bacterium was classified as Pseudomonas pseudoalcaligenes by comparison of its 16S RNA gene sequence to those of existing strains and deposited in the Coleccion Espanola de Cultivos Tipo (Spanish Type Culture Collection) as strain CECT5344. Cyanide consumption is an assimilative p...

The increasing spread of antibiotic-resistant pathogens has raised the interest in alternative antimicrobial treatments. In our study, the functionally active gram-negative bacterium bacteriophage CP933 endolysin was produced in Nicotiana benthamiana plants by a combination of transient expression and vacuole targeting strategies, and its antimicrobial activity was investigated. Expression of t...

abstract nowadays, bypassing agents such as recombinant activated factor vii (rfviia) and activated prothrombin complex concentrates (apcc) are used to treat bleeding episodes in the hemophilia patients with inhibitors. aryoseven® is an iranian biogeneric rfviia with homogeneity of efficacy and the nature to novoseven in a comparative trial. the current clinical trial aimed to evaluate the cost...

histone deacetylase inhibitors have gained a great deal of attention recently for the treatment of cancers and inflammatory diseases. so design of new inhibitors is of great importance in pharmaceutical industries and labs. creating pharmacophor models in order to design new molecules or search a library for finding lead compounds is of great interest. this approach reduces the overall cost ass...

a new series of alkylthio imidazole analogues of captopril, an ace inhibitor used in the treatment of hypertension, was designed and synthesized in order to obtain agents more active than captopril with less side effects. all the compounds thus prepared were purified and characterized by ir, nmr and mass analytical instruments.

objective: alpha-synuclein is a major component of protein plaques in synucleinopathies, particularly parkinson’s disease. the purpose of this study is to assess the inhibitory effects of cuminaldehyde on the fibrillation of alpha-synuclein. methods: alpha-synuclein was expressed in escherichia coli and subsequently purified. for the process of fibrillation, purified protein was incubated at 3...

factors such as oxidative stress and reduced acetylcholine level have been implicated in alzheimer’s disease (ad) pathology and recently there has been a trend towards natural product research to find potential sources of antioxidants and acetylcholinesterase inhibitors in the plants kingdom. centaurea is a genus with about 500 species world wild, many of them have shown to possess biologic act...