Most therapeutic antibodies (Abs) target cell surface proteins on tumor and immune cells. Cloning of Ab gene libraries in E. coli and their display on bacteriophages is commonly used to select novel therapeutic Abs binding target antigens, either purified or expressed on cells. However, the sticky nature of bacteriophages renders phage display selections on cells challenging. We previously repo...

The double membrane system of Gram-negative bacteria is an efficient barrier instrumental in maintaining a steady environment in the cytoplasm. Only a few lowmolecular-mass substances are able to cross this barrier unassisted from the cell exterior. On the other hand, the Gram-negative cell envelope also constitutes a formidable obstacle for proteins destined for the cell exterior. Gram-negativ...

The manipulation of the cell surfaces of prokaryotes (mainly bacteria) and eukaryotes (such as Yeast) has manifested to be an area of stupendous ongoing research, with intelligent widespread applications spanning different arenas of biological sciences (Charbit et al., 1988; Cruz et al., 2000; Francisco et al., 1993; Götz, 1990; Jostock & Dübel, 2005; Keskinkan et al., 2004; Kotrba et al., 1999...

BACKGROUND Cell display technologies (e.g. bacterial display) are attractive in directed evolution as they provide the option to use flow-cytometric cell sorting for selection from combinatorial libraries. The aim of this study was to engineer and investigate an expression vector system with dual functionalities: i) recombinant display of Affibody libraries on Escherichia coli for directed evol...

Homomeric self-assembly of peptides into amyloid fibers is a feature of many diseases. A central role has been suggested for the lateral fiber surface affecting gains of toxic function. To investigate this, a protein scaffold that presents a discrete, parallel β-sheet surface for amyloid subdomains up to eight residues in length has been designed. Scaffolds that present the fiber surface of isl...

Cell surface carbohydrates are important to various bacterial activities and functions. It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved. Here, using atomic force microscopy (AFM)-based recognition force m...

Zwitterionic surfaces are increasingly explored as antifouling coatings due to their propensity resist protein, bacterial, and cell adhesion typically applied polymeric systems. Here, the self-assembly of strongly interacting small molecule amphiphiles is reported produce nanoribbons for applications. Synthesized spontaneously form micrometers-long with nanometer-scale cross-sections intrinsica...

Bacterial cell surface display is a widely used technology for bioadsorption and for the development of a variety of screening systems. Magnetotactic bacteria are unique species of bacteria due to the presence of magnetic nanoparticles within them. These intracellular, nanosized (50 to 100 nm) magnetic nanoparticles enable the cells to migrate and be manipulated by magnetic force. In this work,...