OBJECTIVE To study whether quantitative analysis of M. tuberculosis mRNA could be used to assess bacterial viability. METHODS The levels of M. tuberculosis mRNA were compared 24, 48, and 72 hours after M. tuberculosis H(37)R(V) was treated with no drug, 1.0 micro g/ml and 10.0 micro g/ml rifampicin, 0.2 micro g/ml and 1.0 micro g/ml isoniazid, 2 micro g/ml and 4 micro g/ml ethambutol, 2 micro...

Understanding of soil processes is essential for addressing the global issues of food security, disease transmission and climate change. However, techniques for observing soil biology are lacking. We present a heterogeneous, porous, transparent substrate for in situ 3D imaging of living plants and root-associated microorganisms using particles of the transparent polymer, Nafion, and a solution ...

Drinking water is one of the most valuable resources available to mankind. The presence of pathogens in drinking water is highly undesirable. Because of the Lateritic soil, the iron concentrations were high in ground water. High concentration of iron and other trace elements could restrict bacterial growth and modify their metabolic pattern as well. The bacterial growth rate reduced in the pres...

Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up (3...

abstract edible white button mushroom is a secondary decomposer and the activity of other micro-organisms is necessary to decompose the raw compounds for its growth. actinomycetes bacteria due to their ability to decompose the compound molecules such as cellulose, lingo-cellulose and lignin, play an important role in the composting process for the mushroom. bacterial biomass of actinomycetes co...

Predictive models are mathematical expressions that describe the growth, survival, inactivation, or biochemical processes of foodborne bacteria. During processing of contaminated raw materials and food preparation, bacteria are entrapped into the food residues, potentially transferred to the equipment surfaces (abiotic or inert surfaces) or cross-contaminate other foods (biotic surfaces). Growt...

In this study, we demonstrated a micro-fluidic system with multiple functions, including concentration of bacteria using dielectrophoresis (DEP) and selective capture using antibody recognition, resulting in a high capture efficiency of bacterial cells. The device consisted of an array of oxide covered interdigitated electrodes on a flat silicon substrate and a approximately 16 microm high and ...

A new architecture allowing an increase of the photocurrent of a photovoltaic cell fabricated with a CMOS 0.18μm process is described. This cell has been designed specifically to power the onboard electronics of a 400μm × 600μm untethered microrobot propelled by magnetotactic bacteria (MTB). Four cascaded cells of an area of 200μm × 200μm each, provide a short circuit current of 70μA and an ope...

Using a micro motion picture technique for making records, studies covering several thousand hours of observation have been made of the growth of a number of bacteria. On the basis of these experiments a discussion is offered of bacterial division and its influence on gross colony appearance, of different kinds of pleomorphism that have been observed, and of the nature of the internal structure...

This paper develops a novel approach to microscale locomotion and manipulation using flagellated bacteria for actuation. We use a blotting procedure to attach flagellated bacteria to a buoyancy-neutral plate called a microbarge and deploy the plate in a microchannel . The motion of the plate depends on the propulsion of the bacteria which in turn depends on the distribution of the cells on the ...