meningitis is one of the hazardous and life threatening infections and is associated with mortality and morbidity. the aim of this study was to determine etiological agents of childhood bacterial meningitis. the culture, gram staining, agglutination and pcr assays were used to examine csf specimens from 277 patients with presumed bacterial meningitis for the occurrence of 4 most common infectio...

BACKGROUND Several risk factors for complications after pancreaticoduodenectomy have been reported. However, the impact of intraoperative bacterial contamination on surgical outcome after pancreaticoduodenectomy has not been examined in depth. METHODS This retrospective study included patients who underwent pancreaticoduodenectomy and peritoneal lavage using 7000 ml saline between July 2012 a...

Root and crown rot (gummosis) is known as the most destructive disease affecting pistachio in Iran. The efficiency of bacterial strains to reduce the growth rate of Phytophthora pistaciae was studied under different electrical conductivities (EC, 0, 2, 4, 8, 12 ds/m). Soil and rhizosphere samples were collected from pistachio growing regions in Kerman province, Iran, during 2011 - 2012. Overall...

BACKGROUND Bacterial parapneumonic pleural effusions (PPEs) have high morbidity. The accurate identification of pathogens is vital for initiating the appropriate treatment. A previous study suggested that the use of blood culture bottles might improve the bacterial yield in PPEs. OBJECTIVES The aim of this study was to compare the culture positivity rate by the blood culture bottles and the s...

purpose: to ascertain whether the use of nested-pcr (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of bacterial endophthalmitis. methods: the aqueous humor and vitreous samples of 24 eyes of 24 patients with bacterial endophthalmitis were evaluated by microscopy, diagnostic aerobic culture, and nested- pcr to detect the infectious agent. results: aerobic...

OBJECTIVES To evaluate the value of broad range bacterial PCR in the diagnosis of joint infection and to find out if there are bacteria causing arthritis which are not cultivable by the present methods. METHODS Polymerase chain reaction (PCR) with broad range bacterial primers and DNA sequencing (bacterial PCR) was used to analyse 154 synovial fluid (SF) samples from patients with different a...

OBJECTIVE To investigate bacterial culture practices in veterinary clinics, with an emphasis on laboratory biosafety and on quality of laboratory practices. DESIGN Survey-based prospective study. SAMPLE POPULATION 166 veterinarians. PROCEDURES Veterinarians were recruited through the Veterinary Information Network (an Internet-based network restricted to veterinary personnel). All Network...

We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.