A heparinase that degrades both heparin and heparan sulfate (HS) was purified to homogeneity from the cell-free extract of Bacillus circulans HpT298. The purified enzyme had a single band on SDS-polyacrylamide gel electrophoresis with an estimated molecular mass of 111,000. The enzyme showed optimal activity at pH 7.5 and 45 degrees C, and its activity was stimulated in the presence of 5 mM CaC...

background: punica granatum linn. (pg) is native to the mediterranean region. its flower exhibited antioxidant activity. the present study attempt to investigate the effect of these extract on uterine contraction and its possible mechanism(s). methods: thirty five female wistar rats (200-300 g) at estrous phases of cycle was examined in this study; pieces of virgin adult rat uterus (1.5 cm) wer...

ABSTRACT This paper reports a wavelength-tunable microfluidic optical source for the fluorescence of dye-labeled biochemicals. A pulsed micro-arc is struck between a metal anode and a microfluidic cathode filled with an aqueous solution of BaCl2. Atomic transitions from the Ba atoms, which are sputtered into the glow region, provide strong emissions at 493 nm and 454 nm wavelengths. Undesirable...

This study examined the effect of hyposmotic solutions on the syncytiotrophoblast microvillous membrane potential ( E m) in mature intermediate villi isolated from term human placentas. When villi were exposed to a control solution (280 mosmol/kgH2O; 116 mM NaCl) and then to either a 138-hyposmotic (138 mosmol/kgH2O; 37 mM NaCl) or 170-hyposmotic (170 mosmol/kgH2O; 55 mM NaCl) solution, there w...

The purpose of this study was to use whole-cell and cell-attached patches of cultured skeletal muscle myotubes to study the macroscopic and unitary behavior of voltage-dependent calcium channels under similar conditions. With 110 mM BaCl2 as the charge carrier, two types of calcium channels with markedly different single-channel and macroscopic properties were found. One class was DHP-insensiti...