Low molecular weight glutenin subunits are important components of wheat storage proteins, which play an important role in determining end-use quality of common wheat. A newly established matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) procedure was used to analyze 478 landraces of bread wheat collected from the Yangtze-River region in China. Results ...

Bacillus spp. are well known to protect plants from seed or soil-borne pathogens by the synthesis of various metabolites with antimicrobial activity, such as hy?drolytic enzymes and antibiotics. This study aimed select most effective a group ten antagonistic strains antifungal activity assay. were primarily isolated soil identified B. safensis, pumilus subtilis 16S rDNA sequencing. The four ana...

In the present study we compare genetic characteristics (allele diversity and observed heterozygosity) of microsatellite loci, from three stingless bee species (Plebeia remota Holmberg, Partamona mulata Moure In Camargo and Partamona helleri Friese), amplified by using heterospecific primers originally designed for Melipona bicolor Lepeletier and Scaptotrigona postica Latreille. We analyzed 360...

Polymerase chain reaction (PCR) based RAPD profiles, in conjunction with six primers, of Karnal bunt of wheat and rice bunt exhibiting distinct polymorphic DNA. A total of 84 RAPD loci were observed on polyacrylamide gel for both Tilletia sps. Out of 84, 16 loci were found monomorphic, while other 68 loci were unique. Usefulness of random primers was also checked with other seed borne fungal pa...

In the present study, muscle tissues were obtained separately from individuals from Atlantic hairtail population (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The seven decamer primers were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the three hairtail populations. Here, averagely, a decamer ...

Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evalu...

The genetic diversity of 77 Trichoderma harzianum isolates collected from sunflower rhizosphere soils in Urmia, Khoy, and Salmas West Azerbaijan province, Iran, was evaluated by using the Universal Rice Primer (URP) molecular marker. DNA band pattern developed seven primers this These produced 186 gene loci, out which 182 loci were polymorphic. Accordingly, calculated, their kinship relations d...

We recently developed a multilocus variable-number tandem repeat (TR) analysis (MLVA) assay for Escherichia coli O157:H7 (2). In this assay, we identified seven loci that, when used in combination, were able to identify E. coli O157:H7 outbreaks, discriminate among genetically diverse isolates, and discriminate among isolates that were found to be highly related by pulsed-field gel electrophore...